色综合天天综合欧美综合-精品久久精品久久久久久乐-清纯唯美欧美综合第一页_自拍偷拍亚洲第几页

翌圣生物科技（上海）股份有限

初級會員·13年

聯系人：: 曹女士

電話：: 400-6111-883

手機：

售后：: 4006-111-883

傳真：: 86-21-34615995

地址：: 上海市浦東新區天雄路166弄1號3樓

網址：: www.yeasen.com

在線咨詢給我留言

掃一掃訪問手機商鋪

供應簡介

Fluorescent staining of actin filaments in fixed tissue sections and tissue culture cells preparations. Note: Unlike many actin antibodies, Acti-stain™ 488 phalloidin binds only to F-actin resulting i

詳細介紹

Acti-stain 488 phalloidin 現貨鬼筆環肽綠色熒光同Fitc標記

Product Uses Include

Fluorescent staining of actin filaments in fixed tissue sections and tissue culture cells preparations. Note: Unlike many actin antibodies, Acti-stain™ 488 phalloidin binds only to F-actin resulting in low background fluorescence. Furthermore, binding of F-actin by Acti-stain is not appreciably different between species.

Preparation of stabilized fluorescent actin filaments in vitro.

Actin staining is very useful in determining the structure and function of the cytoskeleton in living and fixed cells. The actin cytoskeleton is a very dynamic and labile structure in the living cell, but it can be fixed with paraformaldehyde prior to probing or staining for actin structures.

Material

Phalloidin is a seven amino acid peptide toxin from the mushroom Amanita phalloides, which binds specifically and with high affinity （Kd 20 nM） to the polymerized form of actin （F-actin）. Phalloidin lowers the critical concentration of actin polymerization to less than 1 µg/ml, thereby acting as a polymerization enhancer. Phalloidin has been labeled with a proprietary green fluorescent dye which allows it to be used to stain actin filaments in tissue cultured cells and tissue sections （1, see Figure 1）?and cell-free preparations. Acti-stain™ 488 phalloidin-labeled actin filaments retain many functional characteristics of unlabeled actin including their ability to interact with myosin. Actin-stain™ 488 phalloidin is supplied as an orange solid.

Note: Phalloidin is toxic and must be handled with care （LD50 human = 2mg/Kg）.

Example Results and Specifications

	Figure 1. Actin Stress Fibers stained with Acti-stain™ 488 in a Swiss 3T3 cell.		Figure 2. Emission and excitation scans for Acti-stain™ phalloidins

	Swiss 3T3 cell stained with anti-vinculin （red）, Dapi （blue nucleus） and F-actin is stained with Acti-stain™ 488 （green F-actin, Cat.# PHDG1）.		Absorbance and fluorescence scan of Acti-stain™ 488. Labeled phalloidin was diluted into methanol and its absorbance and excitation spectra were scanned between 350-750 and 500-750 nm, respectively. Absorbance peaks at 500 nm and fluorescence at 550 nm.

Acti-stain phalloidins are the most well characterized phalloidins available. Tabe 1 describes their brightness, photostability, background and affinity constants to F-actin. Compare these performance characteristics to other fluorescent phalloidins and you will see the advantages of using Acti-stain™ for your actin staining requirements.

Table 1. Biochemical characteristics of fluorescent phalloidins

Conjugate	Cat.#	Wavelengths （Ex/Em）	*Brightness （AFU）**	Stability to photobleaching（half life in seconds）**	Background （% of total AFU at 100nM）**	Affinity （Kd in nanomolar）
Fluorescein-phalloidin	na	485/535 FITC filter set	432	6	22	72 +/-12
Acti-stain™ 488	PHDG1	485/535 FITC filter set	832	57	5	55 +/-8
Acti-stain™ 535	PHDR1	535/585 TRITC filter set	430	27	12	36 +/-7
Acti-stain™ 555	PHDH1	535/585 TRITC filter set	551	46	16	63 +/-8
Acti-stain™ 670	PHDN1	640/680 Cy5 filter set	332	8	18	50 +/-12

* = AFUs measured by quantitative cell imaging. ** = Measured in stained Swiss 3T3 cells in the absence of antifade.

其他相關產品：

產地	貨號	名稱	規格	單價/元	*/元
cytoskeleton	PHDR1	Rhodamine Phalloidin 羅丹明標記現貨	500ul	2100	1785
cytoskeleton	PHDG1-A	Acti-stain™ 488 phalloidin 綠色熒光標記現貨	500u	2436	2070
cytoskeleton	PHDH1-A	Acti-stain 555 phalloidin 紅色熒光標記現貨	500u	2436	2070
cytoskeleton	PHDN1-A	Acti-stain™ 670 phalloidin藍色熒光標記現貨	500u	2436	2070

400-6111-883

實驗室小儀器

分子生物學

蛋白與免疫

細胞生物學

高通量測序建庫

生物醫藥

工具酶

信號轉導

常用生物試劑

化學試劑

耗材

Yeasen/翌圣生物

其他品牌

BioAssay Systems

Polyplus

Thermofisher Scientific/賽默飛世爾

Sigma-Aldrich

會員登錄

收藏該商鋪

提示