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凝血酶，一種由凝血酶前體（血漿中的必要成分）形成的蛋白質水解酶,催化纖維蛋白元變成纖維蛋白而促使血液凝固。

詳細介紹

凝血酶，一種由凝血酶前體（血漿中的必要成分）形成的蛋白質水解酶,催化纖維蛋白元變成纖維蛋白而促使血液凝固。用于毛細血管出血的局部止血以及外科手術后組織愈合。凝血酶能使纖維蛋白原轉化成纖維蛋白。局部應用后作用于病灶表面的血液很快形成穩定的凝血塊，用于控制毛細血管、靜脈出血，或作為皮膚、組織移植物的黏合、固定劑。pH＜5時失效。凝血酶對血液凝固系統的其他作用尚包括誘發血小板聚集及繼發釋放反應等。

別名：Factor IIa;Thrombin;凝血酶

產品描述：

儲存溫度： –20℃

CAS RN：9002-04-4

EC：3.4.21.5

Thrombin is an endolytic serine protease that selectively cleaves the Arg–Gly bonds of fibrinogen to form fibrin and release fibrinopeptides A and B.

The predominant form of thrombin in vivo is the zymogen, prothrombin （factor II）, which is produced in the liver. The concentration of prothrombin in normal human plasma is 5–10 mg/dL.3 Prothrombin is a glycoprotein with a glycan content of ~12%.

Prothrombin is cleaved in vivo by activated factor X, releasing the activation peptide and cleaving thrombin into light and heavy chains yielding catalytically active a-thrombin. a-Thrombin is composed of a light chain （A chain, MW ~6 kDA） and a heavy chain （B chain,MW ~31 kDa）. These two chains are joined by one disulfide bond.4 The B chain of human thrombin consists of a peptide portion （MW 29,485 Da） and a carbohydrate portion （MW 2,334 Da） with N-linked glycosylation at three Asn residues.5,6 Bovine thrombin contains 1.7% glucosamine, 1.8% sialic acid, 0.61% galactose, and 0.95% mannose.

Thrombin also contains g-carboxyglutamyl residues. These modified glutamyl residues are the result of carboxylation by a microsomal enzyme, vitamin Kdependent carboxylase. g-Carboxyglutamyl residues are necessary for the Ca2+-dependent interaction with a negatively charged phospholipid surface, which is essential for the conversion of prothrombin to thrombin.4 Prothrombin is activated in vivo on the surface of a phospholipid membrane that binds the amino terminus of prothrombin along with factors Va and Xa. The activation process starts slowly because factor V is activated to factor Va by the initial, small amount of thrombin.

注意事項及免責聲明：This product is for R&D use only, not for drug, household, or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.

準備說明：The product is soluble in water （10 mg/ml）, yielding a clear solution.

存儲/穩定性：Stock solutions can be prepared at a concentration of 100 units/ml in a 0.1% （w/v） BSA solution. Stock solutions remain active for one week at 0–5℃. Solutions are most stable at pH 6.5, as a pH 7 will greatly reduce thrombin activity. Since thrombin solutions adsorb to glass, it is recommended to aliquot the solutions in plastic tubes and store at –20℃ for long-term storage. Store the lyophilized powder at –20℃. The product retains activity for at least 6 years.

訂購信息：

貨號	名稱	產地	規格	報價	*（元）	庫存
T4648	Thrombin from bovine plasma 凝血酶	Sigma	1000U	648	380	是
T4648	Thrombin from bovine plasma 凝血酶	Sigma	1000U*10	3898	2766	是
	Thrombin from bovine plasma 凝血酶	Mpbio	1KU	422	359	一周內
	Thrombin from bovine plasma 凝血酶	Mpbio	10KU	1246	1060	一周內
30115ES01	Thrombin from bovine plasma 凝血酶（低活力，800 IU/mg）	YEASEN	1 KU	422	359	是
30206ES02	Thrombin from bovine plasma 凝血酶（高活力，>2000 IU/mg）	YEASEN	10 KU	1246	1060	是

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分子生物學

蛋白與免疫

細胞生物學

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