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美國布魯克海文儀器公司>技術(shù)文章>測量應(yīng)用案例-20220208

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測量應(yīng)用案例-20220208

閱讀:132          發(fā)布時間:2022-2-28
 

文獻(xiàn)名: An Electrokinetically-Driven Microchip for Rapid Entrapment and Detection of Nanovesicles

 

 

作者 Leilei Shi 1 and Leyla Esfandiari 1,2,

1 Department of Electrical Engineering and Computer Science, College of Engineering and Applied Sciences, University of Cincinnati, Cincinnati, OH 45221, USA

2 Department of Biomedical Engineering, College of Engineering and Applied Sciences, University of Cincinnati, Cincinnati, OH 45221, USA

 

 

 

摘要:Electrical Impedance Spectroscopy (EIS) has been widely used as a label-free and rapid characterization method for the analysis of cells in clinical research. However, the related work on exosomes (40–150 nm) and the particles of similar size has not yet been reported. In this study, we developed a new Lab-on-a-Chip (LOC) device to rapidly entrap a cluster of sub-micron particles, including polystyrene beads, liposomes, and small extracellular vesicles (exosomes), utilizing an insulator-based dielectrophoresis (iDEP) scheme followed by measuring their impedance utilizing an integrated electrical impedance sensor. This technique provides a label-free, fast, and non-invasive tool for the detection of bionanoparticles based on their unique dielectric properties. In the future, this device could potentially be applied to the characterization of pathogenic exosomes and viruses of similar size, and thus, be evolved as a powerful tool for early disease diagnosis and prognosis.

 

關(guān)鍵詞:nanovesiclebiological nanoparticlesexosomeimpedancedielectrophoresisbiosensingmicrofluidicslab-on-a-chip

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