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abs42024900 百日咳毒素PTX,Pertussis Toxin

參考價(jià) 8000
訂貨量 ≥1
具體成交價(jià)以合同協(xié)議為準(zhǔn)
  • 公司名稱(chēng) 愛(ài)必信(上海)生物科技有限公司
  • 品牌 absin
  • 型號(hào) abs42024900
  • 產(chǎn)地 上海
  • 廠(chǎng)商性質(zhì) 生產(chǎn)廠(chǎng)家
  • 更新時(shí)間 2025/7/29 14:26:21
  • 訪(fǎng)問(wèn)次數(shù) 4925

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愛(ài)必信(上海)生物科技有限公司是一家從事生物學(xué)試劑及耗材的高新技術(shù)企業(yè)。公司秉承“為生命科學(xué)創(chuàng)造便利!為客戶(hù)創(chuàng)造價(jià)值!為員工創(chuàng)造幸福人生!”的發(fā)展理念,擁有專(zhuān)業(yè)的產(chǎn)品研發(fā)質(zhì)檢團(tuán)隊(duì)、市場(chǎng)營(yíng)銷(xiāo)團(tuán)隊(duì)及*倉(cāng)儲(chǔ)物流等管理團(tuán)隊(duì)。榮獲2021年度上海市“專(zhuān)精特新”企業(yè)稱(chēng)號(hào)。

自營(yíng)品牌Absin®,主要經(jīng)營(yíng)產(chǎn)品有:生化試劑(常用生化試劑、生物學(xué)實(shí)驗(yàn)常用試劑、熒光染料、激動(dòng)劑/抑制劑,植物提取物)、細(xì)胞生物學(xué)(細(xì)胞培養(yǎng)產(chǎn)品、血清、基質(zhì)膠、添加劑、檢測(cè)試劑盒)、免疫學(xué)產(chǎn)品、分子生物學(xué)產(chǎn)品、實(shí)驗(yàn)室儀器/耗材、產(chǎn)品定制和檢測(cè)服務(wù)。

Absin還提供一些特色產(chǎn)品:霍亂毒素B亞單位(CTB)、 雞胚提取物(CEE)、 牛腦垂體提取物、 呼吸爆發(fā)試劑盒、 多色免疫組化試劑盒、 mRNA原料等在業(yè)界深受客戶(hù)喜歡。

Absin秉承踏實(shí),務(wù)實(shí),求精和不斷創(chuàng)新的精神為中國(guó)科學(xué)家提供優(yōu)質(zhì)產(chǎn)品,努力打造屬于中國(guó)自己的生物試劑品牌,為廣大科學(xué)家提供更全面更豐富更優(yōu)質(zhì)的產(chǎn)品。

愛(ài)科研,必信它(Absin)!Absin努力成為你身邊的科研百寶箱!







生化試劑,分子生物學(xué)試劑,血清,細(xì)胞培養(yǎng)試劑,化學(xué)試劑,免疫學(xué)試劑,耗材,儀器,定制服務(wù)

CAS 70323-44-3 純度 >99%
分子量 - 分子式 -
供貨周期 現(xiàn)貨 規(guī)格 50ug
貨號(hào) abs42024900 應(yīng)用領(lǐng)域 醫(yī)療衛(wèi)生,環(huán)保,化工,生物產(chǎn)業(yè),農(nóng)林牧漁
主要用途 特定靶向G蛋白抑制G蛋白及其在信號(hào)通路中的生理功能
產(chǎn)品描述
描述

Pertussis Toxin (islet-activating protein), a toxin originally isolated from Bordetella pertussis, has been shown to catalyze ADP-ribosylation of a protein in membrane preparation from rat C6 glioma cells. In addition, exposure of Chinese hamster ovary cells in culture to this compound has resulted in a clustered growth pattern response. Other experiments have shown Pertussis Toxin to block the ability of inhibitory hormones to reduce adenylate cyclase activity and enhance the ability of stimulatory hormones to activate the enzyme. Mechanistic studies have shown this toxin to consist of subunit A (active) promoter, which disrupts transmembrane signaling by ADP-ribosylating eukaryotic G-proteins, and B (binding) oligomer which helps the toxin bind to cells in vivo.

應(yīng)用
A toxin that blocks reduction of adenylate cyclase activity
純度
>99%
活性
Please note that this product is not activated. If your system requires activation, see Kaslow et al.,for suggested conditions.
使用方法
CHO Cell Assay: When examined in a CHO cell assay as described by Hewlett et al.,5 the lowest concentration of toxin at which a positive response (clustered growth pattern) was obtained was 0.01 ng/ml.

Adenylate Cyclase Assay: The adenylate cyclase activity of this lot is 0.67 picomole/min/lJg in the presence of 1 μmolar calmodulin when assayed by the method of Wolff et al6
儲(chǔ)存/保存方法
This product is provided as an aseptically packaged lyophilized powder, sealed under vacuum. Store at 4°C prior to and following reconstitution, for 6 months. DO NOT FREEZE.
基本信息
外觀(guān)
Lyophilized solid
可溶性/溶解性
Soluble in water (Miscible). Reconstitute with sterile purified water or the sterile buffer of your choice. The resulting suspension should be made uniform by gentle mixing prior to use. Do not sterile filter, as this will result in loss of material. For long term storage, reconstitute in a high ionic strength buffer, such as sterile 0.1 M sodium phosphate, pH 7.0, 0.5 M NaCI. Handle the product gently; do not vortex.
參考文獻(xiàn)
參考文獻(xiàn)
1. Bradford, M.M. (1976) Anal. Biochem. 72, 248-254.
2. Tamura, M., Nogomori, K., Murai, S., Yajima, M., Ito, K" Katada, T., Ui, M. and Ishi, S. (1982) Biochem. 21,5516-5522.
3. Wyckoff, M., Rodbard, O. And Chramback, A. (1977) Anal. Biochem.78, 459-482.
4. Laemmli, U.K. (1970) Nature 227, 680-685.
5. Hewlett, E.L., Sauer, K.T., Myers, G.A., Cowell, J.L. and Guerrant, R.L. (1983) Infect. Immun. 40, 1198-1203.
6. Wolff, J., Cook, G.H., Goldhammer, A.R. and Berkowitz, S.A. (1980) PNAS 77, 3841-3844.
7. Kaslow, H.R., Lim, L.-K., Moss, J. and Lesikar, D.O. (1987) Biochem. 26, 123-127.


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