細胞名稱

CCRF-CEM [CCRF CEM]人急性淋巴細胞白血病T淋巴細胞

描述

G.E. Foley 等人建立了類淋巴母細胞細胞株CCRF-CEM。 細胞是1964年11月從一位四歲白人女性急性淋巴細胞白血病患者的外周血白血球衣中得到。

動物種別

人

性別

女

組織來源

白血病, 急性淋巴細胞白血病, 外周血, T淋巴母細胞

形態

淋巴母細胞

培養基和添加劑

添加 2 mM L-谷氨酰胺，10 mM HEPES,1 mM丙酮酸鈉，4.5 g/L 葡萄糖，1.5 g/L 碳酸氫鈉的RPMI 1640， 90%；FBS，10%。.

REFERENCE

Foley GE et al. Continous culture of human lymphoblasts from peripheral blood of a child with acute leukemia. Cancer 18: 522-529, 1965 Sandstrom PA and Buttke TM. Autocrine production of extracellular catalase prevents apoptosis of the human CEM T-cell line in serum-free medium. Proc. Natl Acad. Sci. USA 90:4708-4712, 1993 PubMed: 93281648 Miranda L et al. Isolation of the human PC6 gene encoding the putative host protease for HIV-1 gpl60 processing in CD4+ T lymphocytes. Proc. Natl. Acad. Sci. USA 93: 7695-7700, 1996 PubMed: 96353880 Adams RA. Formal discussion: the role of transplantation in the experimental investigation of human leukemia and lymphoma. Cancer Res. 27: 2479-2482, 1967 PubMed: 68134846 Uzman BG et ah Morphologic variations in human leukemic lymphoblasts （CCRF-CEM cells） after long-term culture and exposure to chemotherapeutic agents. A study with the electron microscope. Cancer 19:1725-I742, 1966 PubMed: 67（146473 Adams RA et al. Leukemia: serial transplantation of human leukemic lymphoblasts in the newborn Syrian halnster. Cancer Res. 27: 772-783. 1967 PubMed: 68122005 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. （1992）, ATCC Quality Control Methods for Cell Lines. 2~a edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1 q88. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D.,（1995） Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Centers for Disease Control （1993）, Biosafety in Microbiological and Biomedical Laboratories Human Health Service Publication No. （CDC） 93-8395. U.S Dept. of Health and Human Services; 3rd Edition U.S. Government Printing Office Washington D.C.