久久国产精品午夜一区_91精品國產高清久久久久久91_?级毛片免费全部播放_欧美色图在线视频一区二区三区_国产综合亚洲一区二区三区_中文字幕熟女网_国产精品男人爽免费视频_九九无码网战_亚洲日本三级电影在线观看_欧美日韩DVD手机在线不卡

上海復(fù)祥生物科技有限公司

當(dāng)前位置:> 供求商機(jī)> 低價(jià)供應(yīng) CRL-1927 SV40 MES 13 小鼠腎小球系膜細(xì)胞

[供應(yīng)]低價(jià)供應(yīng) CRL-1927 SV40 MES 13 小鼠腎小球系膜細(xì)胞

貨物所在地:上海上海市

更新時(shí)間:2025-04-24 21:00:05

有效期:2025年4月24日 -- 2025年10月24日

已獲點(diǎn)擊:113

聯(lián)系我時(shí),請(qǐng)告知來(lái)自 化工儀器網(wǎng)

CRL-1927 SV40 MES 13 小鼠腎小球系膜細(xì)胞,ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|;細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!

CRL-1927 SV40 MES 13 小鼠腎小球系膜細(xì)胞 的詳細(xì)介紹

CRL-1927 SV40 MES 13 小鼠腎小球系膜細(xì)胞

ATCC? Number: CRL-1927?    Price: $355.00
Designations: SV40 MES 13
Depositors:  LJ Striker
Biosafety Level:2 [Cells contain polyomavirus DNA sequences ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties:adherent
Organism:Mus musculus (mouse)
Morphology:myofibroblast-like

Source:Organ: kidney
Tissue: glomerulus
Cell Type: mesangial cell;
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Age: 7 to 10 weeks
Comments:The cells display prominent cytoskeletal staining for actin, and have abundant parallel fibrils in the cytoplasm. They are reported to contract in the presence of 1 X 10(-6) M angiotensin II. The cells form colonies in soft agar, and are positive for SV40 large T antigen.
Propagation: ATCC complete growth medium: The base medium for this cell line is 3:1 mixture of ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002 and Ham's F12 medium with 14 mM HEPES.To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 5%.
Temperature: 37.0°C
Subculturing: Protocol:
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:4 to 1:10 is recommended
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Doubling Time: 26 hrs
Related Products:recommended serum:ATCC 30-2020
References:22754: MacKay K, et al. Glomerular epithelial, mesangial, and endothelial cell lines from transgenic mice. Kidney Int. 33: 677-684, 1988. PubMed: 2835539
51029: Robey RB, et al. Regulation of mesangial cell hexokinase activity by PKC and the classic MAPK pathway. Am. J. Physiol. 277: F742-F749, 1999. PubMed: 10564237
51030: Robey RB, et al. Thrombin is a novel regulator of hexokinase activity in mesangial cells. Kidney Int. 57: 2308-2318, 2000. PubMed: 10844601
61211: Robey RB, et al. Regulation of mesangial cell hexokinase activity and expression by heparin-binding epidermal growth factor-like growth factor: epidermal growth factors and phorbol esters increase glucose metabolism via a common mechanism involving classic mitogen-activated protein kinase pathway activation and induction of hexokinase II expression. J. Biol. Chem. 277: 14370-14378, 2002. PubMed: 11782486
61224: Maile S, et al. The morphology of mesangial cells cultured at high density and in collagen gels. Histol. Histopathol. 15: 403-414, 2000. PubMed: 10809358
61225: Coy PE, et al. LPA is a novel lipid regulator of mesangial cell hexokinase activity and HKII isoform expression. Am. J. Physiol. Renal Physiol. 283: F271-F279, 2002. PubMed: 12110510
90657: Taneja N, et al. Proinflammatory interleukin-1 cytokines increase mesangial cell hexokinase activity and hexokinase II isoform abundance. Am. J. Physiol. Cell Physiol. 287: C548-C557, 2004. PubMed: 15070811


會(huì)員登錄

X

請(qǐng)輸入賬號(hào)

請(qǐng)輸入密碼

=

請(qǐng)輸驗(yàn)證碼

收藏該商鋪

X
該信息已收藏!
標(biāo)簽:
保存成功

(空格分隔,最多3個(gè),單個(gè)標(biāo)簽最多10個(gè)字符)

常用:

提示

X
您的留言已提交成功!我們將在第一時(shí)間回復(fù)您~

以上信息由企業(yè)自行提供,信息內(nèi)容的真實(shí)性、準(zhǔn)確性和合法性由相關(guān)企業(yè)負(fù)責(zé),化工儀器網(wǎng)對(duì)此不承擔(dān)任何保證責(zé)任。

溫馨提示:為規(guī)避購(gòu)買(mǎi)風(fēng)險(xiǎn),建議您在購(gòu)買(mǎi)產(chǎn)品前務(wù)必確認(rèn)供應(yīng)商資質(zhì)及產(chǎn)品質(zhì)量。

撥打電話(huà)
在線(xiàn)留言
主站蜘蛛池模板: 广汉市| 筠连县| 库尔勒市| 新泰市| 门源| 冀州市| 苍梧县| 象山县| 郎溪县| 通化县| 黑山县| 青海省| 石屏县| 和林格尔县| 民县| 苏尼特左旗| 祁东县| 乌鲁木齐市| 洛南县| 清原| 交口县| 大同市| 汨罗市| 文登市| 寿阳县| 林芝县| 合肥市| 新巴尔虎右旗| 长沙市| 杭锦后旗| 绥化市| 三门峡市| 乐亭县| 德兴市| 齐河县| 云浮市| 盘山县| 乐平市| 于田县| 拜泉县| 安化县|