A9 小鼠皮下結締組織細胞
ATCC® Number: CCL-1.4™
Designations: A9 （APRT and HPRT negative derivative of Strain L）
Depositors: TR Chen
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Mus musculus （mouse）
Morphology: fibroblast
Source: Strain: C3H/An
Tissue: subcutaneous connective tissue; areolar and adipose
Disease: normal
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Tumorigenic: Yes
Antigen Expression: H-2k
Cytogenetic Analysis: modal number = 52; range = 46 to 54; This is a hyperdiploid cell line with 24 to 26 marker chromosomes common to all cells., Nine markers （M1 - M9） are common to L-M（TK-） （see ATCC CCL-1.3） and 16 to 17 are found only in A9. The latter markers include Rb（3.5）, Rb（4.15）, Rb（5.5）, T（8;19）, Rb（9.17） and 11 to 12 others., Structurally normal chromosomes 4, 15, 16, 17, 18 and X were absent.
Age: 100 days
Gender: male
Comments: The A9 cell line was isolated by J.W. Littlefield from wild-type Strain L cells.
The cells are sensitive to HAT selection medium and deficient in adenosine phosphoribosyl transferase （APRT） and hypoxanthine phosphoribosyl transferase （HPRT）.
The cells are resistant to 8-azaguanine （0.003 mg/ml） and 6-thioguanine （0.1 mM）.
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5g/L glucose, 90%; fetal bovine serum, 10%
Subculturing: Subc*tion Ratio: A subc*tion ratio of 1:4 to 1:10 is recommended
Medium Renewal: Every 2 to 3 days
Remove old medium, add fresh 0.25% trypsin, EDTA （0.03%） solution, rinse and remove. Allow cells to sit at room temperature （or at 37C） until cells detach （about 5 to 10 minutes）. Add fresh medium, aspirate and dispense into new flasks.
Related Products: parental cell line:ATCC CCL-1
References: 21409: Littlefield JW. The inosinic acid pyrophosphorylase activity of mouse fibroblasts partially resistant to 8-azaguanine. Proc. Natl. Acad. Sci. USA 50: 568-573, 1963. PubMed: 14067106
21410: Chen TR. Cytogenetics of somatic cell hybrids. II. Modal karyotypes and mosaicism in mouse mutant cell lines: LM（tk-）, A9, and Rag. Cancer Genet. Cytogenet. 24: 95-107, 1987. PubMed: 3466673
27669: Mayr GA, Freimuth P. A single locus on human chromosome 21 directs the expression of a receptor for adenovirus type 2 in mouse A9 cells. J. Virol. 71: 412-418, 1997. PubMed: 8985365
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