BNL CL.2 小鼠胚胎肝細胞
ATCC® Number: TIB-73™
Designations: BNL CL.2
Depositors: P Patek, J Collins, M Cohn
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Mus musculus （mouse）
Morphology:
Source: Organ: liver
Strain: BALB/c
Disease: normal
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Tumorigenic: No
Age: embryo
Comments: The line was selected to grow in medium containing ornithine and phenylalanine in place of arginine and tyrosine.
Tested and found negative for ectromelia virus （mousepox）.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:6 is recommended
Medium Renewal: Twice per week
Remove medium, add fresh 0.25% trypsin for 1 minute, remove trypsin and let the culture sit at room temperature until the cells detach （5 to 10 minutes）. Add fresh medium, aspirate and dispense into new flasks.
Related Products: derivative:ATCC TIB-74
References: 22905: Patek PQ, et al. Transformed cell lines susceptible or resistant to in vivo surveillance against tumorigenesis. Nature 276: 510-511, 1978. PubMed: 723934
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