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PT67 is a retrovirus packaging cell line derived from TK- NIH/3T3 cells. The cells produce replication-defective retroviral vector particles capable of binding to the gibbon ape leukemia virus receptor Glvr-1 （Pit-1） or the amphotropic retrovirus receptor Ram-1 （Pit-2） on target cells. [32364]
Propagation:
ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C Atmosphere: air, 95%; carbon dioxide （CO2）, 5%
Subculturing:
Subc*tion Ratio: A subc*tion ratio of 1:4 to 1:12 is recommended Medium Renewal: Every 2 to 3 days Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature （or at 37C） until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation:
culture medium 95%; DMSO, 5%
Related Products:
Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2002 recommended serum:ATCC 30-2020
References:
32364: Miller AD, Chen F. Retrovirus packaging cells based on 10AI murine leukemia virus for production of vectors that use multiple receptors for cell entry. J. Virol. 70: 5564-5571, 1996. PubMed: 8764070 46921: Miller AD. 10A1 Retroviral packaging cells and uses thereof. US Patent 5,766,945 dated Jun 16 1998
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