QQ交談
MSN交談
您所在位置：請輸入產品關鍵字：
郵編：510660
聯系人：楊永漢
電話：86-020-82574011
傳真：86-020-32206070
手機：13802525278
留言：發送留言
個性化：www.jianlun45.com
網址：www.jianlun.com
商鋪：http://www.mcstair.com/st199246/
【產品簡介】
【詳細說明】
NOVABIOS乙腦IGM抗體檢測試劑盒
JE DetectTM IgM ANTIBODY
CAPTURE ELISA (MAC-ELISA)
INTENDED USE
The JE Detect MAC-ELISA test for exposure to Japanese Encephalitis Virus (JEV) is an ELISA assay system for the detection of IgM antibodies in human serum to JEV derived recombinant antigen (JERA) (1-4). This test is to aid in the diagnosis of human exposure to the Japanese Encephalitis Virus (JEV). It is not intended to screen blood or blood components, and is for professional in vitro diagnostic use only.
SUMMARY AND EXPLANATION OF THE TEST
Exposure to JEV causes a disease with a number of symptoms including encephalitis (5-8). The JE Detect MAC-ELISA employs a recombinant antigen called JERA, which can be used as a rapid serological marker for JEV infection. The JERA protein is a recombinant antigen, which consists of a stretch of peptides from different parts of the JEV antigens.
NOVABIOS乙腦IGM抗體檢測試劑盒
PRINCIPLE OF THE TEST
The JE Detect MAC-ELISA consists of one enzymatically amplified "two-step" sandwich-type immunoassay.
In this assay, JE Detect Negative Control (Represents non-reactive serum), JE Detect IgM Positive Control (Represents reactive serum), and unknown serum samples are incubated in microtitration wells which have been coated with anti-human IgM antibodies, followed by incubation with both JEV derived recombinant JERA and Normal Cell Antigen (NCA) separay. The serum samples may be diluted with Sample Dilution Buffer for JE Detect IgM. After incubation and washing, the wells are treated with a JERA-specific antibody labeled with the enzyme horseradish peroxidase (HRP). After a second incubation and washing step, the wells are incubated with the tetramethylbenzidine (TMB) substrate.
An acidic stopping solution is then added and the degree of enzymatic turnover of the substrate is determined by absorbance measurement at 450 nanometers. Above a certain threshold, the ratio of the absorbencies of the JERA and the control wells accuray determines whether antibodies to JEV are present.
更具體的說明請根據以下信息
二維碼掃一掃
【公司名稱】 廣州健侖生物科技有限公司
【】 楊
【】
【騰訊 】 2042552662
【公司地址】 廣州清華科技園創新基地番禺石樓鎮創啟路63號二期2幢101-103室