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Restriction EnzySigma-Aldrich Cloning Expres
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- 上海輔澤商貿有限公司
- 2018-08-17 15:32:00
- 上海市
- 325
【簡單介紹】
| 品牌 | Sigma-Aldrich | 貨號 | Acc I (R6142) |
|---|---|---|---|
| 供貨周期 | 一周 |
【詳細說明】
Restriction Enzyme Buffer Reference
Sigma® Life Science’s restriction enzyme collection has been optimized for digestion using five unique buffers. Reference below the enzyme activity in each buffer (table 1), and the preferred buffer for each enzyme (table 2).
Table 1
Activity (%) of each Sigma restriction enzyme in each Sigma buffer. The preferred buffer for each enzyme is highlighted in bold.
| Buffer | |||||
| Restriction Enzyme | SA (B7531) | SB (B8781) | SL (B3782) | SM (B3158) | SH (B3657) |
| Acc I (R6142) | 100 | 0-10 | 10-25 | 1-10 | 0-10 |
| Alu I (R6885) | 100 | 50-75 | 25-50 | 25-50 | 0-10 |
| Apa I (R4258) | 100 | 10-25 | 50-75 | 50-75 | 0-10 |
| Bam HI (R0260) | 100 | 100 | 75-100 | 100 | 25-50 |
| Bcl I (R8631) | 100 | 100 | 25-50 | 100 | 100 |
| Bgl II (R6377) | 100 | 100 | 25-50 | 100 | 100 |
| Bln I (R3131) | 25-50 | 50-75 | 0-10 | 25-50 | 100 |
| Bsm I (R3635) | 0-10 | 50-75 | 0-10 | 25-50 | 100 |
| Cfo I (R1761) | 75-100 | 50-75 | 100 | 50-75 | 25-50 |
| Cla I (R7763) | 100 | 100 | 75-100 | 100 | 100 |
| Dde I (R4256) | 50-75 | 75-100 | 25-50 | 25-50 | 100 |
| Dpn I (R8381) | 100 | 75-100 | 50-75 | 75-100 | 75-100 |
| Dra I (R4381) | 100 | 75-100 | 100 | 100 | 50-75 |
| Ecl XI (R3884) | 25-50 | 100 | 25-50 | 25-50 | 50-75 |
| Eco RI (R6265) | 100 | 100 | 25-50 | 50-75 | 100 |
| Eco RV (R2756) | 25-50 | 100 | 0-10 | 25-50 | 50-75 |
| Hae III (R5628) | 50-75 | 50-75 | 75-100 | 100 | 25-50 |
| Hind III (R1137) | 50-75 | 100 | 25-50 | 100 | 50-75 |
| Hpa I (R8507) | 100 | 25-50 | 25-50 | 50-75 | 25-50 |
| Kpn I (R1258) | 75-100 | 10-25 | 100 | 25-50 | 0-10 |
| Nco I (R8761) | 50-75 | 50-75 | 50-75 | 50-75 | 100 |
| Nde I (R5509) | 25-50 | 100 | 10-25 | 50-75 | 100 |
| Nhe I (R5634) | 100 | 25-50 | 100 | 100 | 10-25 |
| Not I (R8506) | 10-25 | 50-75 | 0-10 | 25-50 | 100 |
| Nsi I (R5884) | 50-75 | 100 | 10-25 | 50-75 | 100 |
| Pst I (R7023) | 25-50 | 25-50 | 10-25 | 25-50 | 100 |
| Pvu I (R1508) | 50-75 | 75-100 | 25-50 | 50-75 | 100 |
| Pvu II (R2631) | 25-50 | 25-50 | 25-50 | 100 | 25-50 |
| Rsa I (R4756) | 100 | 50-75 | 100 | 50-75 | 0-10 |
| Sac I (R5268) | 100 | 0-10 | 100 | 50-75 | 0-10 |
| Sal I (R0754) | 0-10 | 25-50 | 0-10 | 10-25 | 100 |
| Sau 3A (R0762) | 100 | 25-50 | 25-50 | 75-100 | 0-10 |
| Sca I (R5007) | 0-10 | 100 | 0-10 | 75-100 | 100 |
| Sfi I (R8256) | 25-50 | 25-50 | 75-100 | 100 | 25-50 |
| Sma I (R4503) | 100 | 0-10 | 0-10 | 0-10 | 0-10 |
| Spe I (R5257) | 75-100 | 75-100 | 75-100 | 100 | 100 |
| Sph I (R7135) | 50-75 | 75-100 | 25-50 | 100 | 75-100 |
| Ssp I (R6759) | 75-100 | 75-100 | 10-25 | 75-100 | 100 |
| Taq I (R9507) | 50-75 | 100 | 25-50 | 50-75 | 50-75 |
| Xba I (R7260) | 100 | 75-100 | 75-100 | 75-100 | 100 |
| Xho I (R6759) | 25-50 | 75-100 | 10-25 | 25-50 | 100 |
Table 2
The complete formulation of each Sigma Restriction Enzyme Buffer. The formulation of the storage buffer for each Sigma restriction enzyme is given on the catalog page (see links in table 1).
| Final concentration (mM) | |||||
| SA (B7531) | SB (B8781) | SL (B3782) | SM (B3158) | SH (B3657) | |
| Tris-acetate | 33 | ||||
| Tris-HCl | 10 | 10 | 10 | 50 | |
| Mg-acetate | 10 | ||||
| MgCl2 | 5 | 10 | 10 | 10 | |
| K-acetate | 66 | ||||
| NaCl | 100 | 50 | 100 | ||
| Dithioerythritol (DTE) | 1 | 1 | 1 | ||
| Dithiothreitol (DTT) | 0.5 | ||||
| 2-Mercaptoethanol (BME) | 1 | ||||
| pH at 37°C | 7.9 | 8.0 | 7.5 | 7.5 | 7.5 |
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郵編：200437
聯系人：鄭經理
電話：13816982406,021-65010900
傳真：021-65010900
手機：13816982406
留言：發送留言
網址：
商鋪：http://www.mcstair.com/st215208/