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TRYPSIN, 1-250 胰蛋白酶1:250
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TRYPSIN, 1-250 胰蛋白酶1:250
分子量： Approximay 23,000 or 22000 （for human）
CAS #: 9002-07-7
物理描述：白色至類白色粉末
EC # 3.4.21.4
來源： Human, porcine or bovine pancreas
活性：
One BAEE unit will produce a DA253 of 0.001 per minute at pH 7.6 at 25°C using BAEE as substrate. Reaction volume = 3.2 ml （1 cm light path）.
One TAME unit hydrolyzes 1 umole of p-toluene-sulfonyl-L-arginine methyl ester （TAME） per minute at 25°C, pH 8.2, in the presence of 0.001 M calcium ion.
One USP trypsin unit is the activity causing a change in absorbance of 0.003 per minute under the conditions specified.
Activity Conversion:
1 TAME unit = 19.2 USP or NF units = 57.5 BAEE Units
Composition: Trypsin is composed of two subunits, a-trypsin and b-trypsin. a-Trypsin is composed of two peptide chains and b-trypsin is composed of one chain.
zui適 pH: Approximay 8.0
Extinction coefficient: E1%280 = 14.3
等電點(diǎn)：pH 10.5
抑制劑：Trypsin is inhibited by organophosphorus compounds such as diisopropyl fluorophosphate and natural trypsin inhibitors from pancreas, soybean, lima bean and egg white. Silver ions are also potent inhibitors. Specific inhibitors are AEBSF, antipain, aprotinin, DFP, leupeptin, PMSF, TLCK, and Trypsin Inhibitor.
特異性： The protease activity of trypsin is highly specific toward positively charged side chains with lysine and arginine. Forms complexes with a2-macroglobulin. Can be used in the isolation of intact, detergent-free phycobilisomes and in the hydrolysis/condensation of carboxylic ester bonds.
Solutions: Solutions of trypsin should be thawed and swirled gently to mix. Solutions are relatively stable for periods of up to three months under refrigeration. If longer storage is desired, the concentrate should be aliquoted and refrozen. Thawed concentrate may contain a small amount of precipitate; this is normal and in no way will affect the efficacy of the product.
溶解性： Soluble in water; practically insoluble in alcohol and clycerol.
Formulation （ Trypsin 1:250 in Hanks' Balanced Salt Solution without Magnesium and Calcium）:
CAS #: 9002-07-7
物理描述：白色至類白色粉末
EC # 3.4.21.4
來源： Human, porcine or bovine pancreas
活性：
One BAEE unit will produce a DA253 of 0.001 per minute at pH 7.6 at 25°C using BAEE as substrate. Reaction volume = 3.2 ml （1 cm light path）.
One TAME unit hydrolyzes 1 umole of p-toluene-sulfonyl-L-arginine methyl ester （TAME） per minute at 25°C, pH 8.2, in the presence of 0.001 M calcium ion.
One USP trypsin unit is the activity causing a change in absorbance of 0.003 per minute under the conditions specified.
Activity Conversion:
1 TAME unit = 19.2 USP or NF units = 57.5 BAEE Units
Composition: Trypsin is composed of two subunits, a-trypsin and b-trypsin. a-Trypsin is composed of two peptide chains and b-trypsin is composed of one chain.
zui適 pH: Approximay 8.0
Extinction coefficient: E1%280 = 14.3
等電點(diǎn)：pH 10.5
抑制劑：Trypsin is inhibited by organophosphorus compounds such as diisopropyl fluorophosphate and natural trypsin inhibitors from pancreas, soybean, lima bean and egg white. Silver ions are also potent inhibitors. Specific inhibitors are AEBSF, antipain, aprotinin, DFP, leupeptin, PMSF, TLCK, and Trypsin Inhibitor.
特異性： The protease activity of trypsin is highly specific toward positively charged side chains with lysine and arginine. Forms complexes with a2-macroglobulin. Can be used in the isolation of intact, detergent-free phycobilisomes and in the hydrolysis/condensation of carboxylic ester bonds.
Solutions: Solutions of trypsin should be thawed and swirled gently to mix. Solutions are relatively stable for periods of up to three months under refrigeration. If longer storage is desired, the concentrate should be aliquoted and refrozen. Thawed concentrate may contain a small amount of precipitate; this is normal and in no way will affect the efficacy of the product.
溶解性： Soluble in water; practically insoluble in alcohol and clycerol.
Formulation （ Trypsin 1:250 in Hanks' Balanced Salt Solution without Magnesium and Calcium）:
| Components | mg/L | Mol. Wt. | Mol. （mM） |
| Inorganic Salts | |||
| Potassium Chloride [KCl] | 400.00000 | 74.55 | 5.37 |
| Potassium Phosphate Monobasic [KH2PO4] | 60.00000 | 136.09 | 0.44 |
| Sodium Bicarbonate [NaHCO3] | 350.00000 | 84.01 | 4.17 |
| Sodium Chloride [NaCl] | 8000.00000 | 58.44 | 136.89 |
| Sodium Phosphate Dibasic [Na2HPO4] | 47.50000 | 141.96 | 0.33 |
| Other | |||
| Dextrose | 1000.00000 | 180.2 | 5.55 |
| Trypsin 1:250 | 25000.00000 | n/a | n/a |
訂購(gòu)信息：
| 貨號(hào) | 名稱 | 產(chǎn)地 | 規(guī)格 | 報(bào)價(jià)/元 | */元 |
| | TRYPSIN, 1-250 胰蛋白酶1:250 | MPBIO | 25G | 612 | 248 |
