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GlycanPac AXH-1 Columns for the High-Resolution Separation and Analysis of Glycans
The Thermo Scientific™ GlycanPac™ AXH-1 column is a high-performance, silica-based HPLC column for simultaneous separation of glycans by charge, size, and polarity. It is designed for high-resolution and high-throughput analysis with unique selectivity for biologically important glycans, either labeled or native, by LC-fluorescence and LC-mass spectrometry (MS) methods.
The GlycanPac AXH-1 column can be used for qualitative, quantitative, and structural characterization of uncharged and charged glycans present in biological molecules (e.g., proteins).
Features:
Unique glycan selectivity based on charge, size, and polarity
Excellent resolution for both native and labeled glycans
Useful for both high-resolution glycan profile characterization and easy quantification of glycans based on charge
Compatible with fluorescence and MS detection methods
High chromatographic efficiency and excellent column stability
The GlycanPac AXH-1 column, based on innovative mixed-mode surface chemistry, combines both weak anion-exchange (WAX) and hydrophilic interaction liquid chromatography (HILIC) retention mechanisms for optimal selectivity and resolution power. The WAX functionality provides retention and selectivity for negatively charged glycans, while the HILIC mode facilitates the separation of glycans of the same charge according to their polarity and size. As a result, the GlycanPac AXH-1 column provides unparalleled capabilities for glycan separations. Additionally, this column offers charge-based separations for various glycans without discrimination of size and polarity, making it a suitable tool for accurate quantification of glycans which cannot be achieved with other HPLC columns. The GlycanPac AXH-1 column is designed and tested for LC-fluorescence detection and LC-MS applications using volatile aqueous buffers (e.g., ammonium acetate or ammonium formate) and acetonitrile.
GlycanPac AXH-1 columns are based on high-purity and spherical silica substrates in both 1.9 and 3 µm sizes for UHPLC and standard HPLC applications, respectively. GlycanPac AXH-1 columns are available in various dimensions for applications with different resolutions, throughput, and instrument requirements.
| GlycanPac AXH-1 Column Specifications | |
|---|---|
| Column Dimensions | 1.9 μm: 2.1 × 250mm, 2.1 × 150mm, 2.1 ×100mm 3 μm: 4.6 × 150mm, 3.0 × 150mm, 2.1 × 150mm |
| Bead Size | Spherical 1.9 μm, 3 μm particles |
| Substrate | High-purity, Porous, Spherical Silica |
| Functional Group | WAX and HILIC Mixed-Mode |
| Recommended Flow Rate Range: | 1.9 μm: 0.1 to 0.4 mL/min. for: all 2.1 mm columns 3 μm: 0.6 to 1.2 ml/min for 4.6 × 150 mm 0.3 to 0.6 ml/min for 3.0 × 150 mm 0.1 to 0.5 ml/min. for 2.1 × 150 mm |
| Pressure Limit | 1.9 μm: 7,000 psi for 2.1 × 100mm, 10,000 psi for 2.1 ×150mm 15,000 psi for 2.1 × 250mm 3 μm: 6,000 psi for all 3 μm columns |
| Temperature Range: | Ambient to 60 ºC |
| pH Range | 2.0 - 8.0 |