傳統的2D培養系統無法支持放大培養（Scale-up）所需細胞數量級別（> 10⁹個細胞/處理）以維持細胞療法的增長需求。 3D懸浮培養是大規模生產多能細胞的可行經濟有效的解決方案。盡管干細胞傳統上難以適應懸浮培養，但CellArtis研究人員已經開發出一種培養體系，可以在成分確定、無飼養層和無異源物條件下實現穩定高效的生長并維持多能性。Cellartis DEF-CS 500 iPS無外源3D懸浮培養基與生物反應器系統配套，為創建大量高質量，未分化的起始材料提供了理想的方法。該培養基的配方基于2D培養基Cellartis DEF-CS 500 Xeno-Free Culture Medium，2D培養的細胞可以輕松過渡到3D以實現可靠的放大。

3D懸浮培養基特點

球體中的多能細胞保留*分化潛能和穩定的核型

優化用于下游定向分化的iPS細胞放大

傳代3-4次，在1L生物反應器中產生5x10⁹個細胞

優勢在于擴增細胞達到治療所需細胞數量

建議細胞培養規格

細胞接種量	錐形培養瓶大小	培養體積
1–4 x 10⁶	25 ml	5–15 ml
3–8 x 10⁶	100 ml	16–30 ml
7–20 x 10⁶	250 ml	35–65 ml

組份：基礎培養基+三種添加劑

Cellartis DEF-CS 500 Xeno-Free 3D Spheroid Culture Medium w/o Antibiotics (Cat. No. Y30047)

500 ml Cellartis DEF-CS 500 Xeno-Free Basal Medium w/o Antibiotics (Cat. No Y30046; not sold separay)

Cellartis DEF-CS 500 Xeno-Free 3D Spheroid Additives (Cat. No. Y30048)

o 500 µl DEF-CS Xeno-Free 3D Spheroid Additive 1 (1,000X)

o 200 µl DEF-CS Xeno-Free 3D Spheroid Additive 2 (4,000X)

o 400 µl DEF-CS Xeno-Free 3D Spheroid Additive 3 (500X)

另行購買試劑：

緩沖液和細胞分離試劑

PBS Dulbecco's w/o Ca2+ & Mg2+ (D-PBS –/–)

Accumax (Merck Millipore, Cat. No. SCR006)

可選抗生素和消化試劑

Gentamicin (Thermo Fisher, Cat. No. 15750)

Versene Solution (Thermo Fisher, Cat. No. 15040) (for transfer from monolayer culture)

英文描述

Cellartis DEF-CS 500 Xeno-Free 3D Spheroid Culture Medium

Traditional 2D culture systems simply cannot support the scale-up required (>109 cells/treatment) to sustain the growing demand for cell-based therapies. 3D suspension culture is a viable, cost-effective solution for large-scale production of pluripotent cells. Although stem cells have traditionally been difficult to adapt to suspension culture, our scientists have developed a culture system that allows robust, stable growth and maintenance of pluripotency under defined, feeder-free, and xeno-free conditions. The Cellartis DEF-CS 500 Xeno-Free 3D Spheroid Culture Medium paired with a perfusion bioreactor system provides an ideal method for creating large amounts of high-quality, undifferentiated starting material. The formulation of this medium is based on the 2D medium, Cellartis DEF-CS 500 Xeno-Free Culture Medium, and cells growing in 2D can easily be transitioned to 3D for robust scale-up.

DEF-CS™ 500 Xeno-Free 系列培養基

Y30045 DEF-CS™ 500 Xeno-Free 培養基：從基礎研究過渡到臨床前研究

Y30047 DEF-CS™ 500 Xeno-Free 3D 懸浮培養基：獲取大量高質量的細胞治療材料

Y30071 DEF-CS™ 500 Xeno-Free GMP 級別培養基：遵循嚴格的GMP生產制造標準

華雅再生醫學旗艦公司：紅榮微再（上海）生物工程技術有限公司主營細胞治療和精準醫療等產品，2018年與TAKARA繼續合作，*代理（上海）其旗下Cellartis干細胞研究制品和Rubicon單細胞文庫構建試劑。Cellartis公司位于瑞典，擁有iPS細胞等干細胞分化為肝細胞及臟器細胞的分化誘導技術和ES細胞，ips細胞，分化細胞等干細胞相關產品。

手機1500 1904 520 紅榮微再-客服經銷商專員

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細胞治療

耗材

細胞凍存/細胞分離

細胞庫

干細胞（無血清）培養基

血清及替代物/細胞因子

抗體/蛋白質

核酸分析/測序/蛋白組學

小型儀器設備

檢測試劑

其他

微球

生長因子/細胞因子

PCR試劑耗材

NGS測序試劑耗材

[供應]Cellartis Y30047 DEF-CS 500 Xeno-Free 3D Spheroid Culture Medium