久久国产精品午夜一区_91精品國產高清久久久久久91_?级毛片免费全部播放_欧美色图在线视频一区二区三区_国产综合亚洲一区二区三区_中文字幕熟女网_国产精品男人爽免费视频_九九无码网战_亚洲日本三级电影在线观看_欧美日韩DVD手机在线不卡

上海橋星貿(mào)易有限公司

主營(yíng)產(chǎn)品: 進(jìn)口透析袋,密理博ECL發(fā)光液,B27無(wú)血清培養(yǎng)基,Gibco膠原酶

11

聯(lián)系電話

18221794820

您現(xiàn)在的位置: 首頁(yè)> 技術(shù)文章 > 免疫印跡與檢測(cè)技術(shù)指南

進(jìn)口透析袋/透析膜現(xiàn)貨

美國(guó)進(jìn)口透析袋(D)

Gibco—Invitrogen試劑

生物試劑進(jìn)口

50ml離心管架耐高壓

進(jìn)口96孔黑色酶標(biāo)板

Gibco原裝膠原酶

胎牛血清

B27無(wú)血清添加劑

Trizol LS Gibco

發(fā)光底物HRP

12800-017DMEM高糖培養(yǎng)基

聯(lián)合碳化透析袋

干型透析袋大卷

USA便捷型透析袋(MD)

Spectrumlabs即用型透析袋

生物實(shí)驗(yàn)耗材

透析袋夾子

美國(guó)MFPI透析袋

微生物培養(yǎng)基

PeproTech細(xì)胞因子

Invitrogen反轉(zhuǎn)錄酶III

精細(xì)化學(xué)試劑

自產(chǎn)試劑

Biosharp*生物科技

通用生化試劑

Elisa試劑盒

細(xì)胞培養(yǎng)基

Sigma試劑

Formumax|氯氟松|巨噬細(xì)胞清除劑

亞科因

橋星自營(yíng)品牌

公司信息

聯(lián)人:
蔣經(jīng)理
話:
021-65672052
機(jī):
18221794820
真:
86-021-62097628
址:
上海市楊浦區(qū)寧國(guó)路229號(hào)13號(hào)樓704室
編:
200090
個(gè)化:
www.qx147.com
網(wǎng)址:
www.qx5147.com
鋪:
http://www.mcstair.com/st321209/
給他留言

免疫印跡與檢測(cè)技術(shù)指南

2016-4-20 閱讀(3443)

Overview of Western Blotting

The term "blotting" refers to the transfer of biological samples from a gel to a membrane and their subsequent detection on the surface of the membrane. Western blotting (also called immunoblotting because an antibody is used to specifically detect its antigen) was introduced by Towbin, et al. in 1979 and is now a routine technique for protein analysis. The specificity of the antibody-antigen interaction enables a target protein to be identified in the midst of a complex protein mixture. Western blotting can produce qualitative and semiquantitative data about that protein.

Introduction

The first step in a Western blotting procedure is to separate the macromolecules using gel electrophoresis. After electrophoresis, the separated molecules are transferred or blotted onto a second matrix, generally a nitrocellulose or polyvinylidene difluoride (PVDF) membrane. Next, the membrane is blocked to prevent any nonspecific binding of antibodies to the surface of the membrane. Most commonly, the transferred protein is complexed with an enzyme-labeled antibody as a probe. An appropriate substrate is then added to the enzyme and together they produce a detectable product such as a chromogenic precipitate on the membrane for colorimetric detection. The most sensitive detection methods use a chemiluminescent substrate that, when combined with the enzyme, produces light as a byproduct. The light output can be captured using film, a CCD camera or a phosphorimager that is designed for chemiluminescent detection. Alternatively, fluorescently tagged antibodies can be used, which are directly detected with the aid of a fluorescence imaging system. Whatever system is used, the intensity of the signal should correlate with the abundance of the antigen on the membrane.

Detailed procedures for detection of a Western blot vary widely. One common variation involves direct vs. indirect detection. With the direct detection method, the primary antibody that is used to detect an antigen on the blot is labeled with an enzyme or fluorescent dye. This detection method is not widely used as most researchers prefer the indirect detection method for a variety of reasons. In the indirect detection method, a primary antibody is added first to bind to the antigen. This is followed by a labeled secondary antibody that is directed against the primary antibody. Labels include biotin, fluorescent probes such as fluorescein or rhodamine, and enzyme conjugates such as horseradish peroxidase or alkaline phosphatase. The indirect method offers many advantages over the direct method.

免疫印跡與檢測(cè)技術(shù)指南相關(guān)產(chǎn)品:

發(fā)光底物HRP(ECL發(fā)光液)50ml*2密理博WBKLS0100現(xiàn)貨
發(fā)光底物HRP(ECL發(fā)光液)250ml*2密理博WBKLS0500現(xiàn)貨
Pierce ECL免疫印跡底物皮爾斯32106現(xiàn)貨
增強(qiáng)型化學(xué)發(fā)光(ECL)HRP底物皮爾斯34080現(xiàn)貨

 



產(chǎn)品對(duì)比 產(chǎn)品對(duì)比 二維碼 在線交流

掃一掃訪問(wèn)手機(jī)商鋪

對(duì)比框

在線留言
主站蜘蛛池模板: 任丘市| 开原市| 调兵山市| 宁都县| 广州市| 离岛区| 岫岩| 柘荣县| 张家界市| 尼木县| 彝良县| 赣州市| 革吉县| 察雅县| 深州市| 天峨县| 肇州县| 伊川县| 平乐县| 古交市| 高州市| 乌兰察布市| 米泉市| 巴青县| 突泉县| 高密市| 兴安盟| 湛江市| 秭归县| 富源县| 安泽县| 老河口市| 祁连县| 涡阳县| 旬邑县| 滦南县| 龙南县| 嘉兴市| 舟山市| 尉犁县| 新泰市|