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Product Introduction:Amine-activated agarose beads can be used for the immobilization of carboxyl containing or aldehyde containing molecules. The coupling chemistry is carbodiimide-based and is a successful and well-documented technique. Amine-activated SepFast forms a chemically stable amide linkage with ligands containing carboxyl groups. It has a long spacer (9 atoms). The pre-activated agarose base matrix can be readily employed to make various custom affinity chromatography adsorbents for both small scale and large scale purification applications.
Product Properties:
Amine-activated SepFast 4 High Flow (4HF) is made of highly cross-linked 4% beaded agarose. It shows high mechanical rigidity allowing high flow throughput with reduced back pressure.
Agarose has long been used for chromatographic separations due to its excellent hydrophilic and low non-specific-binding nature. The particles have an open pore structure with excellent mass transfer properties to large protein molecules.
The base matrix is activated by attaching a long hydrophilic chain with an amine group in the end. They react with either carboxyl containing ligands in the presence of a carbodiimide agent, or aldehyde containing ligands in the presence of a reducing agent. Amine-activated SepFast is supplied as an aqueous suspension in 20% ethanol.
Group to be coupled | -COOH, CHO |
Matrix | Highly cross-linked 4% agarose beads (4HF) |
Particle size | 50 – 150 µm |
Particle size | 50 – 150 µm |
Spacer arm | 9 atoms |
Activation level | 10 - 25 µmol amine / ml medium |
pH stability | 2 -13 (ligand dependent) |
Chemical stability | Compatible with all commonly used aqueous chemicals, provided the ligand to be coupled can withstand |
Storage | +4℃—+8℃ |
Product Series:
Product | Quantity | Code no. |
Amine-activated SepFast 4HF | 5 ml | 350101-5ML |
50 ml | 350101-50ML | |
1 Litre | 350101-1L | |
Amine-activated SepFast 6HF | 5 ml | 350301-5ML |
50 ml | 350301-50ML | |
1 Litre | 350301-1L |
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