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AACT抗胰糜蛋白酶（兔多克隆抗體）

α-1-Antichymotrypsin 

廣州健侖生物科技有限公司

AACT是一種絲氨酸蛋白酶抑制劑，可以中和糜蛋白酶等酶活性的蛋白酶，存在于大多數的組織細胞、巨噬細胞以及多種胃腸道和肺部腫瘤中，但多形核白細胞中無此物質，它可以作為組織細胞瘤和良性/惡性纖維組織細胞瘤的標志。此抗體與人的AACT反應，主要用于惡性纖維組織細胞瘤等惡性腫瘤的診斷,也可用于胃癌、肺癌、腎癌等腫瘤的研究。

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【產品介紹】

細胞定位：細胞漿

適用組織：石蠟/冰凍

陽性對照：扁桃體

抗原修復：熱修復（EDTA）

抗體孵育時間：30-60min

AACT抗胰糜蛋白酶（兔多克隆抗體）

剛開始嘗試“培養”視網膜時，我們實驗室還在探討視網膜形成的一些基本問題。我們知道，視網膜是從胎兒大腦中名為“間腦”(diencephalon)的那一部分發育而來的。在胚胎發育的早期階段，間腦的一部分會擴展，形成氣球狀的視泡(optic vesicle)，后者再向內凹陷，形成視杯;視杯進一步形變，zui終成為視網膜。
一個多世紀以來，生物學家一直就視杯形成的精確機制爭論不休，直到今天，研究大腦發育的科學家仍然各執一詞。其中一個較有爭議的問題是，在視杯形成過程中，與之相鄰的一些結構，如晶狀體和角膜起了什么作用?有些科學家認為，視網膜向內凹陷，是因為受到了晶狀體的物理推動作用;也有科學家認為，視杯無須借助晶狀體的作用，就可以自己形成。
要想在活著的、正在發育中的動物身上觀察這一現象絕非易事，因此大約在10年前，我的研究團隊決定做一次嘗試，看能不能把眼睛的發育過程“提取”出來。
具體做法是，先在培養皿中培養胚胎干細胞，然后加入眼睛發育所需的化學物質，觀察培養皿中發生的情況。從發育程度上來說，胚胎干細胞是zui原始的干細胞，zui終可以分化成從神經到肌肉的各種組織。
當時，把干細胞培育成器官的技術尚不存在。人們曾把相互分離的干細胞“撒”在膀胱或食管形狀的人工骨架上，試圖搭建出新的器官。這類組織工程學技術在培植真實器面并不是很成功。
因此，我們決定另辟蹊徑。在正式動手之前，我們做了一些準備工作。2000年，我們發明了一種細胞培養方法，可以把小鼠的胚胎干細胞轉變成多種神經細胞。隨后，我們在培養皿中培養了一層小鼠胚胎干細胞，并加入一些可充當“傳遞員”的細胞——這些細胞會向胚胎干細胞傳遞化學信號，促使后者發育、分化，脫離胚胎狀態。我們培養這些細胞的目的，并不是要復制某個人體器官的三維結構，而是想看看，僅用細胞自身的化學信號，是否足以讓胚胎干細胞形成眼睛發育早期所*的神經細胞。
起初，我們沒有獲得多大的成功，但在2005年，我們在技術上取得了突破。以前，我們實驗室在培養干細胞時，細胞只能平鋪在培養皿上，但在2005年，我們突破了“二維限制”，可以讓干細胞懸浮在培養液中，這就是“懸浮培養”。我們采用這種三維培養技術的原因有很多。首先，在懸浮培養中，細胞聚集時，本身就會形成三維結構，因此在產生復雜組織時，會比平鋪的細胞層更容易;其次，為了發育成復雜的結構，細胞之間需要相互交流，而三維培養更適于促進這樣的交流，因為細胞之間可以更加靈活地發生相互作用。
使用這種新方法，我們把相互分離的胚胎干細胞懸浮在液體培養基中，然后注入多孔培養皿的小孔中(每個小孔只有微量的培養基，含大約3 000個胚胎干細胞)。我們發現，原本分開的胚胎干細胞開始聚集在一起。

我司還提供其它進口或國產試劑盒：登革熱、瘧疾、流感、A鏈球菌、合胞病毒、腮病毒、乙腦、寨卡、黃熱病、基孔肯雅熱、克錐蟲病、違禁品濫用、肺炎球菌、軍團菌、化妝品檢測、食品安全檢測等試劑盒以及日本生研細菌分型診斷血清、德國SiFin診斷血清、丹麥SSI診斷血清等產品。

想了解更多的產品及服務請掃描下方二維碼：

【公司名稱】 廣州健侖生物科技有限公司
【市場部】    楊永漢

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【騰訊  】 
【公司地址】 廣州清華科技園創新基地番禺石樓鎮創啟路63號二期2幢101-103室

At the beginning of trying to "c*te" the retina, our laboratory is still exploring some of the basic problems of retinal formation. We know that the retina develops from that part of the fetal brain called diencephalon. In the early stages of embryonic development, a portion of the diencephalon expands to form a balloon-shaped optic vesicle, which in turn sinks inwardly to form an optic cup; the optic cup further deforms to eventually become the retina.
For more than a century, biologists have been arguing for the exact mechanism by which cups are formed. Until today, scientists studying brain development remained silent. One of the more controversial issues is the role of adjacent structures such as the lens and cornea during optic cup formation. Some scientists believe that the retina is inwardly depressed because of the physical impetus of the lens Role; also some scientists believe that the cup without the help of the role of lens, you can form their own.
Observing this phenomenon on living, developing animals is by no means an easy task, so about 10 years ago my team decided to make an attempt to "extract" the development of the eye.
This is done by first culturing embryonic stem cells in a petri dish and then adding the chemicals needed for eye development to observe what is happening in the petri dish. In terms of development, embryonic stem cells are the most primitive stem cells that eventually differentiate into various tissues ranging from nerve to muscle.
At the time, the technology to grow stem cells into organs did not exist yet. People have "sprinkled" separated stem cells on the artificial skeleton of the bladder or esophagus in an effort to build new organs. Such tissue engineering techniques are not very successful in developing real organs.
Therefore, we decided to find another way. Before we started, we made some preparations. In 2000, we invented a cell culture method that transforms mouse embryonic stem cells into a variety of nerve cells. We then cultured a layer of mouse embryonic stem cells in a Petri dish and added cells that act as "transferees" - these cells send chemical signals to the embryonic stem cells that cause the latter to develop, differentiate, and detach themselves from the embryo. Instead of trying to copy the three-dimensional structure of a human organ, we want to see if the chemical signals of the cells alone are enough for embryonic stem cells to become neurons that are unique to early eye development.
At first, we did not get much success, but in 2005, we made a technological breakthrough. In the past, when we cultured stem cells in our laboratory, the cells were only laid on the culture dish. However, in 2005, we broke through the "two-dimensional limitation" and allowed the suspension of stem cells in the culture medium. This is called "suspension culture." There are many reasons why we use this three-dimensional culture technique. First, in suspension culture, cells accumulate and form three-dimensional structures themselves, making them easier to produce complex tissues than tiled cell layers. Second, cells need to communicate with each other in order to develop complex structures , While three-dimensional c*tion is better suited to facilitate such exchanges because the cells can interact more flexibly.
Using this new method, we suspended the embryonic stem cells isolated from each other in liquid medium and injected them into the wells of a multi-well culture dish (with only a minimal amount of media per well containing approximay 3,000 embryonic stem cells). We found that originally separated embryonic stem cells began to congregate.