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流感熒光定量RT-PCR診斷試劑盒：日本富士（瑞必歐）、日本生研、美國BD、美國NovaBios、美國binaxNOW、英國clearview、凱必利、廣州創侖等。歡迎大家，廣州健侖生物科技有限公司
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流感熒光定量RT-PCR診斷試劑盒
廣州健侖生物科技有限公司
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廣州健侖長期供應各種流感檢測試劑，包括進口和國產的品牌，主要包括日本富士瑞必歐、日本生研、美國BD、美國NovaBios、美國binaxNOW、英國clearview、凱必利、廣州創侖等主流品牌。
主要檢測：甲型流感病毒檢測試劑、乙型流感病毒檢測試劑、甲乙型流感病毒檢測試劑、A+B流感病毒檢測試劑盒、流感病毒抗原快速檢測卡、流感病毒抗體快速檢測試劑盒、流感快速檢測試劑 c1c2。
流感熒光定量RT-PCR診斷試劑盒
實時熒光定量PCR （Quantitative Real-time PCR）是一種在DNA擴增反應中，以熒光化學物質測每次聚合酶鏈式反應（PCR）循環后產物總量的方法。通過內參或者外參法對待測樣品中的特定DNA序列進行定量分析的方法。·
Real-timePCR是在PCR擴增過程中，通過熒光信號，對PCR進程進行實時檢測。由于在PCR擴增的指數時期，模板的Ct值和該模板的起始拷貝數存在線性關系，所以成為定量的依據。
所謂實時熒光定量PCR技術[1] ，是指在PCR反應體系中加入熒光基團，利用熒光信號積累實時監測整個PCR進程，zui后通過標準曲線對未知模板進行定量分析的方法。
檢測方法
1.SYBRGreenⅠ法：
在PCR反應體系中，加入過量SYBR熒光染料，SYBR熒光染料特異性地摻入DNA雙鏈后，發射熒光信號，而不摻入鏈中的SYBR染料分子不會發射任何熒光信號，從而保證熒光信號的增加與PCR產物的增加*同步。
SYBR定量PCR擴增熒光曲線圖
PCR產物熔解曲線圖（單一峰圖表明PCR擴增產物的單一性）
2.TaqMan探針法：
探針完整時，報告基團發射的熒光信號被淬滅基團吸收；PCR擴增時，Taq酶的5’-3’外切酶活性將探針酶切降解，使報告熒光基團和淬滅熒光基團分離，從而熒光監測系統可接收到熒光信號，即每擴增一條DNA鏈，就有一個熒光分子形成，實現了熒光信號的累積與PCR產物的形成*同步。
1. TaqMan熒光探針：PCR擴增時在加入一對引物的同時加入一個特異性的熒光探針，該探針為一寡核苷酸，兩端分別標記一個報告熒光基團和一個淬滅熒光基團。探針完整時，報告基團發射的熒光信號被淬滅基團吸收；PCR擴增時，Taq酶的5'－3'外切酶活性將探針酶切降解，使報告熒光基團和淬滅熒光基團分離，從而熒光監測系統可接收到熒光信號，即每擴增一條DNA鏈，就有一個熒光分子形成，實現了熒光信號的累積與PCR產物形成*同步。而新型TaqMan-MGB探針使該技術既可進行基因定量分析，又可分析基因突變（SNP），有望成為基因診斷和個體化用藥分析的*技術平臺。
2. SYBR熒光染料：在PCR反應體系中，加入過量SYBR熒光染料，SYBR熒光染料非特異性地摻入DNA雙鏈后，發射熒光信號，而不摻入鏈中的SYBR染料分子不會發射任何熒光信號，從而保證熒光信號的增加與PCR產物的增加*同步。SYBR僅與雙鏈DNA進行結合，因此可以通過溶解曲線，確定PCR反應是否特異。
3. 分子信標：是一種在5和3末端自身形成一個8個堿基左右的發夾結構的莖環雙標記寡核苷酸探針，兩端的核酸序列互補配對，導致熒光基團與淬滅基團緊緊靠近，不會產生熒光。PCR產物生成后，退火過程中，分子信標中間部分與特定DNA序列配對，熒光基因與淬滅基因分離產生熒光。
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流感熒光定量RT-PCR診斷試劑盒
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【公司名稱】 廣州健侖生物科技有限公司
【市場部】 歐
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【騰訊 】
【公司地址】 廣州清華科技園創新基地番禺石樓鎮創啟路63號二期2幢101-103室
Quantitative real-time PCR (Quantitative Real-time PCR) is a method of measuring the total amount of product after each PCR reaction with a fluorescent chemical in a DNA amplification reaction. A method for quantitative analysis of a specific DNA sequence in a test sample by internal or external parameters. ·
Real-time PCR is a real-time PCR process that uses fluorescence signals during PCR amplification. Since there is a linear relationship between the Ct value of the template and the initial copy number of the template during the PCR amplification exponential phase, it becomes a quantitative basis.
The so-called real-time fluorescence quantitative PCR technology [1], refers to the PCR reaction system by adding fluorescent groups, the use of fluorescence signal accumulation in real-time monitoring of the entire PCR process, and finally through the standard curve of quantitative analysis of unknown template method.
Detection method
1.SYBR Green I method:
In the PCR reaction system, an excess of SYBR fluorescent dye is added, and the SYBR fluorescent dye is specifically doped into the DNA double-stranded and emits a fluorescent signal, while the SYBR dye molecules not incorporated into the chain do not emit any fluorescence signal, thereby ensuring the fluorescence signal The increase is compley synchronized with the increase in PCR product.
SYBR quantitative PCR amplification fluorescence curve
PCR product melting curve (single peak graph shows the PCR amplification product of the unity)
2. TaqMan probe method:
When the probe is intact, the fluorescent signal emitted by the reporter group is absorbed by the quencher group. During the PCR amplification, the 5'-3 'exonuclease activity of the Taq enzyme degrades the probe so that the fluorescent group and the quencher Eliminate the fluorescence group separation, so fluorescence monitoring system can receive the fluorescence signal, that is, for each amplification of a DNA chain, there is a fluorescent molecule formation, the accumulation of fluorescence signals and the formation of PCR products compley synchronized.
1. TaqMan Fluorescent Probe: A pair of primers is added to a specific fluorescent probe during PCR amplification. The probe is an oligonucleotide, labeled with a reporter fluorescent group and a quencher Fluorescent groups. When the probe is intact, the fluorescent signal emitted by the reporter group is absorbed by the quencher group. During the PCR amplification, the 5'-3 'exonuclease activity of the Taq enzyme degrades the probe so that the fluorescent group and the quencher Eliminate the fluorescence group separation, so fluorescence monitoring system can receive the fluorescence signal, that is, for each amplification of a DNA chain, there is a fluorescent molecule formation, the accumulation of fluorescence signal and the PCR product is compley synchronized. The new TaqMan-MGB probe enables both quantitative gene analysis and genetic mutation (SNP) analysis and is expected to be the technology of choice for gene diagnosis and individualized drug use analysis.
2. SYBR Fluorescent dye: SYBR fluorescent dye is added to PCR reaction system, SYBR fluorescent dye non-specific incorporation of DNA double-stranded, fluorescence signal is emitted, but not incorporated into the chain of SYBR dye molecules will not emit any fluorescence Signal, thereby ensuring that the increase of the fluorescence signal is compley synchronized with the increase of the PCR product. SYBR binds only to double-stranded DNA, so you can determine if the PCR reaction is specific by lysing the curve.
3. Molecular Beacons: A stem-ring double-labeled oligonucleotide probe that forms a hairpin structure of about 8 bases by itself at the ends 5 and 3, and the nucleic acid sequences at both ends are complementary-matched to each other, resulting in the interaction of the fluorophore with Quenched groups tightly close, will not produce fluorescence. After the PCR product is generated, during annealing, the middle part of the molecular beacon is paired with the specific DNA sequence, and the fluorescent gene is separated from the quencher gene to generate fluorescence