二、使用Zeta Life胎牛血清Z7186FBS-500培養 3T3-L1小鼠胚胎成纖維細胞(前脂肪細胞)；
三、文章簡介：乳糖酶抑制劑 通過下調脂肪形成 JAK2 / STAT3信號通路介導的有絲分裂.
四、文章部分內容見下：
Cell Culture and Adipocyte Differentiation Induction
3T3-L1 preadipocytes obtained from American Type Culture Collection (ATCC) were maintained in DMEM (SH30022.01, HyClone, CT, USA) containing 10% fetal bovine serum (Z7186FBS-500, ZETA LIFE, Menlo Park, CA, USA). To induce differentiation, 2-day post confluent 3T3-L1 cells (designatedat day 0) were cultured in DMEM containing 10% FBS, 0.5 mM 3-isobutyl-1-methylxanthine (IBMX)(I7018, Sigma-Aldrich, St. Louis, MO, USA), 1 µM dexamethasone (D4902, Sigma-Aldrich, MO, USA),and 10 µg/mL insulin (91077C, Sigma-Aldrich, St. Louis, MO, USA) for two days. Cells were thenmaintained in DMEM containing 10% FBS and 10 µg/mL insulin for the rest of the culture.
產品名稱
貨號
規格
Fetal Bovine Serum France Origin
Z7186FBS-500
500ml
Fetal Bovine Serum France Origin
Z7186FBS-100
100ml
Fetal Bovine Serum ,South America Origin
Z7181FBS-500
500ml
Fetal Bovine Serum ,South America Origin
Z7181FBS-100
100ml
Fetal Bovine Serum (South America Origin) Ultra-low Endotoxin
Z7180FBS-500
500ml
Fetal Bovine Serum (South America Origin) Ultra-low Endotoxin
Z7180FBS-100
100ml
Fetal Bovine Serum (Australia Origin）
Z7010FBS-500
500ml
Fetal Bovine Serum (Australia Origin）
Z7010FBS-100
100ml
Fetal Bovine Serum ,South America Origin
Z7185FBS-500
500ml
Fetal Bovine Serum ,South America Origin
Z7185FBS-100
100ml
Zeta Life介紹
美國 Zeta Life 公司成立于 1989 年，是無血清細胞培養基 DMEM/F12 主要完成人，有三十多年的胎牛血清、無血清細胞培養基、以及干細胞、免疫細胞及腫瘤細胞等多種不同類型哺乳動物細胞的無血清培養液生產經驗；也是目前胎牛血清、無血清培養基較大的 OEM 供應商；每批血清均有完整的血源證明文件、獸醫師檢驗證明及品質測試報告；ZETA LIFE 血清在 100 級的無菌間采用全自動方式制造生產，具有良好的可再現性及可追溯性。
2018 年美國 Zeta Life 公司與美國加利福尼亞大學舊金山校區聯合開發活細胞、活體動物體內可代謝的 Advanced DNA RNA 轉染試劑，此技術成為蛋白功能、免疫細胞及干細胞治療、研發及生產的主要關鍵技術.