當前位置:上海富雨生物科技有限公司>>細胞庫 / 細胞培養>>普通細胞>> NR8383大鼠肺泡巨噬細胞(
大鼠肺泡巨噬細胞NR8383
屬 | 大鼠 |
別稱 | NR-8383; NR 8383; NR8383.1; AgC11x3A; Normal Rat, August 3, 1983 |
組織來源 | 肺;肺泡 |
疾病 | 自發永生細胞系 |
傳代比例/細胞消化 | 1:2傳代,懸浮部分離心收集(1000RPM,5分鐘) ,貼壁部分消化1-2分鐘 |
培養基配置 | Ham's F-12K培養基; 20%胎牛血清;1%雙抗 |
簡介 | NR8383細胞來源于肺灌洗時的正常大鼠肺泡巨噬細胞 ,NR8383細胞在Gerbil肺細胞連續培養液存在下培養了8-9個 月。隨后 ,NR8383細胞不再需要外源生長因子。通過有限稀釋法 ,從單個細胞克隆并亞克隆NR8383細胞 ,并3次用 軟瓊脂亞克隆。NR8383細胞表現出巨噬細胞的特性:吞噬酵母多糖和銅綠、非特異性脂酶活性、Fc受體、氧化降 解;分泌IL-1、TNF-β和IL-6 ,可重復地響應外源生長因子。NR8383細胞響應 ,分泌TNF-β前體。 NR8383細胞在刺激下 ,TNF-β mRNA表達也上升。NR8383細胞對內毒素敏感 ,1-10ng/ml的LPS水平抑 制增生達50%。即使達到0.001mg/ml的水平 ,LPS抑制還是無毒且在后續過程中可逆的。NR8383細胞提供了高響 應的肺泡巨噬細胞的均一來源 ,可以用于體外研究巨響細胞相關活性 |
形態 | 巨噬細胞樣 |
生長特征 | 貼壁 ,懸浮混合生長 |
倍增時間 | 每周 2 至 3 次 |
培養條件 | 氣相:空氣 ,95% ;二氧化碳 ,5%。 溫度:37攝氏度 ,培養箱濕度為70%-80%。 |
凍存條件 | 凍存液:90%FBS ,DMSO 10%, 或使用非程序凍存液:貨號JY-H040 |
保藏機構 | ATCC; CRL-2192 |
備注 | 該細胞為半懸浮和半貼壁細胞 ,懸浮細胞離心收集 ,貼壁細胞消化處理 |
產品使用 | 于科學研究 ,不可作為動物或人類疾病的治療產品使用。 |
cowpea-wheat crop sequence. Appl. Soil Ecol. 157, 103764
Galvez, A., Sinicco, T., Cayuela, g.L., gingorance, g.D., Fornasier, F., gondini, C.,
atientswithAISofditerentseverities were compared , andthecorrelationbetween serum OccludinlevelsandNIHSSscoresin p atientswith AIS was
as a p redictorofAISoccurrence , hemorrha g ictransformation and p oor p ro g nosis .
Keywords:Acuteischemicstroke ; Occludin; Bloodbrainbarrier
Both resistance training (RT) and long-duration, high-intensity stretching induce muscular adaptations; however, it is unknown whether the modalities are complementary or redundant, particularly in well-trained individuals. A case-study was conducted on a competitive bodybuilder implementing long-duration, high-intensity stretching of the plantar flexors (60?min 6x/week for 12?weeks) in conjunction with their habitual RT. Ultrasound muscle architecture (muscle thickness [MT], fascicle length [FL], and pennation angle [PA]) measurements were collected at multiple sites at four weekly baseline sessions, six (mid) and 12 (post1) weeks following the commencement of the intervention, and a week after the intervention (post2) while isometric strength and range of motion (RoM) were obtained once at baseline, mid, post1, and post2. 2SD band plots were constructed to determine meaningful changes in MT, FL, and PA from the four baseline measures while percentage and absolute change across each timepoint were calculated for all variables. From baseline to post 1, RoM, strength, and MT increased 25.9%, 11.4%, and 7.4%-23.4%, respectively, while four MT and two PA sites exceeded the threshold for meaningful change. The combined stretching and RT protocols resulted in flexibility, strength, and MT adaptations; however, findings should be generalized with caution given the case-study nature of our investigation.
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