PriCells: Assay of the vascular endothelial permeability in mice                     

Mice were housed in a pathogen-free environment.
Mice (6-8 weeks old, 18-22 g) were injected intravenously with Chicago sky blue dye 60 B (100 µL of 10 mg/mL) through the tail vein
Mice were killed 2 hours and the abdominal skin was removed.
Photographs of the abdominal skin were taken with a digital Sony Cyber 3.2-megapixel camera.
Images were processed with Adobe Photoshop 6.0 software.
For the measurement of microvascular permeability in multiple organs, mice were injected with Chicago blue dye 60B (100 µL of 10 mg/mL) through the tail vein.
At 30 minutes after dye injection, mice were killed and tissues (abdominal skin, lungs, heart, liver, and intestine; 0.1 g) were removed and put into 0.6 mL of 0.5% Na^₂SO^₄ and 1.4 mL of acetone.
The dye was extracted at 55°C for 24 h, and the absorbance measured at 630 nm using Revelation Microsoft in an MRX microplate reader (DYNEX Technologies).

The vascular permeability index for each mouse was determined by dividing the absorbance value.