This cell line was generated from the T Cell Receptor (TCR) Knockout NFAT Luciferase Reporter Jurkat Cell Line (BPS Bioscience #78556) by overexpression of human CD8 (NM_001768.6) and NY-ESO-1 TCR (c259) using lentiviral transduction (CD8a Lentivirus #78648 and NY-ESO-1 -Specific TCR Lentivirus Clone c259 #78676). The human TCR clone c259 that specifically recognizes antigen NY-ESO-1 (New York esophageal squamous cell carcinoma 1).

Figure 1: Illustration of the functional co-culture assay used to validate the NY-ESO-1 TCR (c259) CD8⁺ NFAT-Luciferase Reporter Jurkat cell line.

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