The Kynurenine 3-Monooxygenase (KMO) Inhibitor Screening Assay Kit is designed to measure human KMO enzyme inhibition. The KMO Inhibitor Screening Assay Kit is simple to use. Recombinant human KMO is mixed with the test inhibitor and the reaction is initiated through addition of substrates NADPH and L-Kynurenine. After incubation, KMO activity is determined by measuring the absorption of the reaction product at λ=340 nm. The UV absorption signal correlates with the amount of NADPH remaining in the reaction, therefore the signal is inversely related to the enzymatic activity.