The FcRn:HSA Binding Chemiluminescent Assay Kit is designed for screening and profiling small molecule inhibitors or peptides that can block the interaction between Human Serum Albumin (HSA) and human FcRn. This kit comes in a convenient 96-well format, with purified Biotinylated FcRn (FCGRT/B2M) (amino acids 24-297 of FCGRT and 21-119 of B2M) and HSA proteins, Streptavidin-HRP, and assay buffers for 100 reactions.

The assay requires only a few steps. First, HSA is coated on a 96-well plate overnight. After blocking, the protein is pre-incubated with the inhibitor. Upon subsequent incubation with Biotin-FcRn, the plate is treated with Streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can then be measured using a chemiluminescence reader.