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Highlights
•CTS HiFi Cas9 Protein can reduce over 90% of off-targeteffects in primary T cells compared to CTS TrueCutCas9 Protein (wild-type Cas9)
• CTS HiFi Cas9 Protein can maintain equivalent knockoutand editing efficiencies compared to CTS TrueCut Cas9Protein across several tested targets
• Increase in HDR % can be achieved when usingCTS HiFi Cas9 Protein with CTS Xenon GenomeEditing Buffer
• Consistent high performance was also achieved in stemcells with CTS HiFi Cas9 Protein