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含有 GlutaMAX 添加劑的 Gibco RPMI 1640 培養基含有穩定形式的 L-，在細胞培養時不會自發降解，可以防止氨沉積，改善細胞健康狀況并提高細胞活性。

RPMI 1640 培養基含有還原劑、、12 以及 PABA，這些維生素是 Eagle 必需培養基和 Dulbecco 改良 Eagle 培養基所不具備的。此外，RPMI 1640 培養基含有高濃度維生素肌醇和。由于 RPMI 1640 培養基不包含蛋白質、脂質或生長因子，因此通常需要補充胎牛血清（FBS）。RPMI 1640 培養基使用的緩沖系統需要在5–10%的 CO₂ 環境條件下來維持生理 pH 值。

賽默飛世爾科技針對多種細胞培養應用，對 RPMI 培養基組分進行了大量的改良。

RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium) was originally developed to culture human leukemic cells in suspension and as a monolayer. RPMI 1640 medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of RPMI 1640 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

The complete formulation is available.

Using RPMI 1640 Medium
RPMI 1640 medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 medium contains biotin, vitamin B₁₂, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. RPMI 1640 medium with GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format. RPMI 1640 medium contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 medium uses a sodium bicarbonate buffer system (2.0 g/L) and therefore requires a 5–10% CO₂ environment to maintain physiological pH.