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Gibco Advanced RPMI 1640 培養(yǎng)基包含的成分可減少50-90%的 FBS 添加劑，這既能降低成本，又能減少批次間血清的變化，提高細(xì)胞培養(yǎng)的一致性。

Gibco RPMI 1640 培養(yǎng)基被發(fā)現(xiàn)適用于多種哺乳動(dòng)物細(xì)胞，包括 HeLa、Jurkat、MCF-7、PC12、PBMC、星形膠質(zhì)細(xì)胞和癌細(xì)胞。

Advanced RPMI (Roswell Park Memorial Institute) 1640 is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation. Compared to classic RPMI 1640, serum supplementation can be reduced by 50-90% with no change in growth rate or morphology.ⁱ Cells successfully cultured in Advanced RPMI 1640, with no adaptation, include Sp2, Vero, Raji, Daudi, and Jurkat.

The complete formulation is available.

Gibco™ Advanced RPMI 1640 is unique from other media due to addition of the following ingredients to allow for serum reduction: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, AlbuMAX™ I lipid-rich bovine serum albumin for cell culture, and the trace elements sodium selenite, ammonium metavanadate, cupric sulfate, and manganous chloride.

Product Use
For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.

Advanced RPMI 1640 requires supplementation with 1-5% Fetal Bovine Serum and 4 mM L-glutamine or GlutaMAX™ supplement. Many cell lines do not require adaptation to this media. The FBS concentration must be optimized for each cell line to obtain maximum serum reduction. Advanced RPMI 1640 uses a sodium bicarbonate buffer system (3.7 g/L) and therefore requires a 5-10% CO₂ environment to maintain physiological pH.

ⁱAdvanced Media: Reduced Sera Solutions. Quest, 2004. 1.3:26-30.