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| 產(chǎn)品英文名稱 | IPKine™ Anti-GFP Magnetic IP Kit |
| 產(chǎn)品中文名稱 | IPKine™ GFP標(biāo)簽蛋白免疫沉淀試劑盒（磁珠法） |
| 免疫原 | 合成多肽 |
| 試劑盒組分 | Non-Denaturing Lysis Buffer TBS (10×) Anti-GFP Magnetic Beads Mouse IgG Magnetic Beads Elution Buffer Neutralization Buffer SDS-PAGE Loading Buffer (5×) |
| 特點(diǎn)&優(yōu)勢(shì) | ? 高效：特異性強(qiáng)、靶蛋白結(jié)合量高，≥0.6 mg GFP標(biāo)簽融合蛋白/mL磁珠； ? 通用：提供IP實(shí)驗(yàn)所需的所有必要緩沖液； ? 可靠：提供陰性對(duì)照，可排除IgG本身和目的蛋白或其它特定生物分子的非特異性結(jié)合； ? 靈活：本試劑盒提供兩種洗脫方法（酸洗脫和SDS-PAGE Loading Buffer洗脫方法）。 |
| 保存建議 | 按各組分標(biāo)簽提示分開(kāi)存儲(chǔ)，保質(zhì)期12個(gè)月。 |
| 運(yùn)輸條件 | 冰袋運(yùn)輸（藍(lán)冰） |
| 警告 | 本文列出的產(chǎn)品僅供研究使用，不適用于人類或臨床診斷。我們產(chǎn)品所推薦應(yīng)用，不是建議使用我們的產(chǎn)品去違反任何或許可證。對(duì)于使用本產(chǎn)品可能發(fā)生的侵權(quán)或其他違規(guī)行為，我們不承擔(dān)任何責(zé)任。 |
| 背景 | The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9kD) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish. The GFP has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum. |
Figure. The immunoprecipitation effect of Anti-GFP Magnetic IP Kit used for GFP-Tag fusion protein. HEK293T cells were transfected with GFP-Tag plasmid. Lane 1 was whole cell lysate (WCL); Lane 2 was the immunoprecipitation sample of Mouse IgG Magnetic Beads eluted by 1×SDS-PAGE Loading Buffer; Lane 3 was the immunoprecipitation sample of Anti-GFP Magnetic Beads eluted by 1×SDS-PAGE Loading Buffer. Lane 4 was the immunoprecipitation sample of Anti-GFP Magnetic Beads eluted by Elution Buffer. By using acid elution, only contained GFP-Tag fusion protein, did not contain heavy and light chains of antibody.
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