化工儀器網
技術文章
測量應用案例-20220301
閱讀：141 發布時間：2022-3-24文獻名： Efficient magnetic harvesting of microalgae enabled by surface-initiated formation of iron nanoparticles
作者： Xiaojie Liabc, Bin Liuac, Yongmin Laoac, Peng Wanac, Xuemei Maoac, Feng Chenac
a Shenzhen Key Laboratory of Marine Microbiome Engineering, Institute for Advanced Study, Shenzhen University, Shenzhen 518060, China
b Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, China
c Institute for Innovative Development of Food Industry, Shenzhen University, Shenzhen 518060, China
摘要：Magnetic separation represents a cost-effective and energy-saving strategy for harvesting targets of interest, e.g., small molecules, biomacromolecules, and cells, in complex mixture. Conventional approaches necessitate multi-step time-consuming processes, including the pre-synthesis of magnetic nanoparticles and subsequent surface modification with targeting ligands. Here, a biocompatible and rapid separation strategy was developed for six microalgal species by in situ formation of iron nanoparticles on the microalgal surface. This method streamlines the separation process of various species of microalgae (e.g., Chlorella zofingiensis) from culture medium with a high harvesting efficiency (greater than 95%) within 1 min spanning a wide pH range of 4–11. Moreover, the recovered microalgae showed intact viability and exhibited no deterioration effect on the downstream extraction process of high-value bioproducts (e.g., chlorophyll, carotenoids and lipids). This method not only provides an alternative to harvest microalgae in a facile manner, but also demonstrates a new pathway to separate other microorganisms and cells, providing more opportunities in economic bioengineering and bioresource production.
關鍵詞：Magnetic harvesting；Chlorella zofingiensis；Microalgae；Iron nanoparticles；In situ synthesis