久久国产精品午夜一区_91精品國產高清久久久久久91_?级毛片免费全部播放_欧美色图在线视频一区二区三区_国产综合亚洲一区二区三区_中文字幕熟女网_国产精品男人爽免费视频_九九无码网战_亚洲日本三级电影在线观看_欧美日韩DVD手机在线不卡

文獻名： Preparation, characterization and in vitro photodynamic therapy of a pyropheophorbide-a-conjugated Fe3O4 multifunctional magnetofluorescence photosensitizer

 

作者： Jianjun Cheng,^a   Guanghui Tan,^ab   Wenting Li,^a   Jinghua Li,^a   Zhiqiang Wang^a and    Yingxue Jin^a  

^a Key Laboratory for Photonic and Electronic Bandgap Materials, Ministry of Education, College of Chemistry & Chemical Engineering, Harbin Normal University, Harbin, China

^b College of Life Science and Technology, Harbin Normal University, Harbin, China

 

 

摘要：Novel pyropheophorbide-a-conjugated multifunctional magnetofluorescence nanoparticles Fe3O4@SiO2@APTES@Glutaryl-PPa (MFNPs) with a mean diameter of 50 nm were strategically designed and prepared for photodynamic therapy (PDT) and medical fluorescence imaging. Chlorin photosensitizer pyropheophorbide-a (PPa) was covalently anchored on the surface of core–shell Fe3O4@SiO2@APTES nanoparticles that were prepared via a sol–gel process with a bridging glutaryl group. The phase constitution, morphology, size, chemical properties, magnetic property of the intermediates and final nanoparticles were characterized by X-ray powder diffraction, transmission electron microscopy, Fourier transform infrared spectrometer, zeta potential, vibration sample magnetometer, thermogravimetric analysis, ultraviolet-visible absorption spectra and fluorescent emission spectroscopy. These results showed that the MFNPs have good dispersibility in alcohol and water, excellent magnetization with 17.31 emu g−1 at 300 K, strong superparamagnetic and good photoluminescence property. The in vitro PDT against the human HeLa cervical cancer cell suggested that MFNPs could permeate the tumor cells quickly and possess suitable lipo-hydro partition coefficient, inducing damage and apoptotic cell death. The cancer cell viability was lowered to 10.18% after treatment with PDT. In addition, the formation of reactive oxygen species in HeLa cells after MFNPs-PDT treatment was studied, which suggested that Type I and Type II photodynamic reactions can occur simultaneously.