活性氧（ROS）和氧化應(yīng)激可能是多種疾病（如糖尿病、癌癥）發(fā)生發(fā)展的關(guān)鍵因素，并且可能在衰老過(guò)程中發(fā)揮重要作用。8-羥基-2'-脫氧鳥苷（8-OHdG）是由羥基自由基、單線態(tài)氧和直接光動(dòng)力作用形成的氧化損傷DNA的產(chǎn)物。8-OHdG可以在組織、血清、尿液和其他生物材料中被檢測(cè)到?？?span style="font-family: Calibri;">8-OHdG單克隆抗體（克隆N45.1）對(duì)DNA損傷具有高度特異性，不會(huì)與RNA氧化產(chǎn)物（如8-羥基鳥嘌呤和8-羥基鳥苷）發(fā)生交叉反應(yīng)。適用于免疫組化和ELISA檢測(cè)。

經(jīng)過(guò)4周慢性紫外線B照射后，無(wú)毛小鼠的表皮用N45.1染色。

（Y. Hattori等人：《實(shí)驗(yàn)皮膚病學(xué)雜志》107卷，第733-737頁(yè)，1996年）

艾美捷JaICA抗8-OHdG單克隆抗體（N45.1）：

貨號(hào)：MOG-020P

克隆號(hào)： N45.1

抗原： 8-OHdG-鑰孔血藍(lán)蛋白

亞類： 小鼠IgG1（kappa）

制備方式： 以腹水形式制備，并用硫酸銨純化。

形式： 凍干粉

包裝： 每瓶含20或100微克IgG（分別為MOG-020P和MOG-100P）。

特異性： 19種8-OHdG類似物{鳥苷（G）、7-甲基-G、6-SH-G、8-溴-G、dA、dC、dT、dI、dU、dG、O6-甲基-dG、8-OHdA、鳥嘌呤（Gua）、O6-甲基-Gua、8-OH-Gua、尿酸、尿素、肌酸、肌酐}均未顯示交叉反應(yīng)性。僅8-巰基-G和8-OH-G表現(xiàn)出極小的交叉反應(yīng)性（小于1%）。

用途： 免疫組化和ELISA

儲(chǔ)存： -20°C以下可保存5年

運(yùn)輸： 在室溫下至少穩(wěn)定7天。

文獻(xiàn)參考：

1)S.Toyokuni, T.Tanaka, Y.Hattori, Y,Nishiyama, A.Yoshida, K.Uchida, H.Hiai, H.Ochi, and T.Osawa: Quantitative immunohistochemical determination of 8-hydroxy-2'deoxyguanosine by a monoclonal antibody N45.1: Its application to ferric nitrilotriacetate-induced renal carcinogenesis model. Lab. Invest. 76(3), p365-374 (1997)

[ Specifity of the antibody clone N45.1.]

2)Y.Hattori, C.Nishigori, T.Tanaka, K.Uchida, O.Nikaido, T.Osawa, H.Hiai, S.Imamura, and S.Toyokuni: 8-Hydroxy-2'deoxyguanosine is increased in epidermal cells of hairless mice after chronic ultraviolet B exposure. J. Invest. Dermatol. 107, p733-737 (1996)

[ Immunohistochemistry of UV-B irradiated mouse skin. ]

3)T.Tanaka, Y.Nishiyama, K.Okada, K.Hirota, M.Matsui, J.Yodoi, H.Hiai, and S.Toyokuni: Induction and nuclear translocation of thioredoxin by oxidative damage in the mouse kidney: independence of tubular necrosis and sulfhydryl depletion. Lab. Invest. 77(2), p145-155 (1997)

[ Immunohistochemistry of ferric nitrotriacetate-treated mouse kidney. ]

4)Y.Ihara, S.Toyokuni, K.Uchida, H.Odaka, T.Tanaka, H.Ikeda, H.Hiai, Y.Seino, and Y.Yamada: Hyperglycemia causes oxidative stress in pancreatic -cells of GK rats, a model of type 2 diabetes. Diabetes 48, p927-932 (1999)

[ Immunohistochemistry of pancreatic islets from GK rat, a diabetes model. ]

5)T. Ichiseki, A. Kaneuji, S. Katsuda1, Y. Ueda,T. Sugimori and T. Matsumoto: DNA oxidation injury in bone early after steroid administration is involved in the pathogenesis of steroid-induced osteonecrosis. Rheumatology 44,p456-460(2005)

[ Immunohistochemistry of rabbit tissue. ]

6)Lee YA, Cho EJ, Yokozawa T: Protective effect of persimmon (Diospyros kaki) peel proanthocyanidin against oxidative damage under H2O2 -induced cellular senescence. Biol Pharm Bull 31(6)p1265-1269(2008)

[ Application to human cultured cells. ]