I. INTRODUCTION
Human IGF-I ELISA （Enzyme-Linked Immunosorbent Assay） kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human IGF-I in serum, plasma, cell culture supernatants and urine （this kit only can detect a free form of human IGF-I）. This assay employs an antibody specific for human IGF-I coated on a 96-well plate. Standards and samples are pipetted into the wells and IGF-I present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human IGF-I antibody is added. After washing away unbound biotinylated antibody, HRPconjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in
proportion to the amount of IGF-I bound. The Stop Solution changes the color from blue to yellow, Human IGF-I ELISA  Kit and the intensity of the color is measured at 450 nm.

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