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MAbPac Protein A Column for Monoclonal Antibody Titer Analysis
The Thermo Scientific™ MAbPac™ Protein A column is a high-performance, affinity chromatography column specifically designed for fast, accurate determination of monoclonal antibody (MAb) concentration in the harvest cell culture (HCC). Early in the development of recombinant MAbs, a large of number of HCC samples must be screened for IgG titer. Affinity chromatography employing a Protein A ligand is often used to determine the MAb concentration. The MAbPac Protein A column is a high-pressure liquid chromatography (HPLC) column designed to provide fast, accurate, titer analysis with linearity over a wide concentration range without carryover.
The MAbPac Protein A column is based on a novel nonporous polymeric resin consisting of a hydrophobic divinylbenzene core and a hydrophilic surface. This proprietary resin is designed for fast mass transfer, resulting in efficient separations without carryover. The recombinant Protein A ligands are covalently attached onto the hydrophilic resin surface through their amine groups to provide a very rugged, reproducible column. The column is available in a HPLC bioinert, 4 × 35 mm PEEK column body.
Product Highlights:
• A novel nonporous polymeric resin with a hydrophilic surface
• Functionalized with the recombinant protein A ligands
• Wide range of MAb quantitation: 0.025–5 mg/mL
• Fast analysis: 2 min/analysis
• Rugged
• Low pressure: column pressure is less than 250 psi at 2 mL/min
The MAbPac Protein A column can be used for both titer analysis or concentrating and purifying IgG material for later experiments or two-dimensional (2D) chromatography.
| MAbPac Protein A Column Specifications | |
|---|---|
| Column Dimension | 4 × 35 mm |
| Base Matrix Material | Hydrophilic nonporous resin |
| Surface Ligand | Protein A |
| Particle Size | 12 µm |
| Binding capacity | ~3.5 mg IgG/g resin |
| Dynamic Loading Capacity | ≤100 µg IgG/column at 2 mL/min flow rate |
| pH Range | 1.9–7.5 |
| Maximum Flow rate | Up to 2 mL/min |
| Maximum Pressure | ≤1000 psi |
| Operating Temperature | <30 ?C |