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Ideal Column for the Separation of Neutral and Acidic Monosaccharides
The CarboPac® PA1 is an anion-exchange column for the separation of mono-, disaccharides, and specific oligosaccharides using an isocratic eluent. Sialic acid analysis has also been demonstrated on this column.
General-purpose column for isocratic determination of monosaccharides and disaccharides
Detection by pulsed amperometric detection; no derivatization needed
Approved for use in a variety of official methods for the analysis of foods
The unique MicroBead™ pellicular structure of the CarboPac PA1 resin gives it stability from pH 0–14 at all concentrations of buffer salts. The pellicular resin structure allows excellent mass transfer resulting in fast gradient reequilibration.
The CarboPac PA1 column is used with pulsed amperometric detection; no derivatization is required. Sensitivity on the PA1 is not as high as with the CarboPac PA20 or PA10 for monosaccharide analysis. Thus the CarboPac PA1 is well suited for the analysis of foods for nutritional labeling. The CarboPac PA1 has been approved for use in a number of official methods.
| CarboPac PA1 Column for Routine Carbohydrate Analysis Specifications | |
|---|---|
| Dimensions | CarboPac PA1 Analytical Column: 4 × 250 mm and 2 × 250 mm CarboPac PA1 Guard Column: 4 × 50 mm and 2 × 50 mm CarboPac PA1 Semipreparative Column: 22 × 250 mm |
| Maximum operating pressure | 4000 psi (27.9 MPa) |
| Temperature range | 4–55 °C Recommended operating temperature: ambient |
| Typical operating conditions | 1500 psi at 1.0 mL/min (analytical and guard columns) Recommended flow rate: 1.0 mL/min (for 4 × 250 mm) |
| phase compatibility | pH 0–14; up to 2% of common HPLC solvents. Acetate or hydroxide eluents only. |
| Resin composition | 10-µm-diameter substrate (polystyrene 2% cross-linked with divinylbenzene) agglomerated with 500-nm MicroBead quaternary ammonium functionalized latex (5% cross-linked). |
| Anion-exchange capacity | Approximay 100 µeq/column (4 × 250 mm analytical column) |
| Column construction | PEEK with 10-32 threaded-ferrule-style end fittings. All components are nonmetallic. |