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For Specialized Amino Acid Applications with Postcolumn Derivatization
The AminoPac® PA1 column is a high-speed, pellicular, strong, anion-exchange column. The PA1 delivers high-efficiency separations as a result of its nonporous-core particle. Its strong anion-exchange characteristics are imparted by agglomerating surface MicroBead™ particles that are functionalized with the quaternary ammonium functional group, resulting in a higher capacity than typically offered by nonporous resins.
Resolves phosphorylated and other amino acids by anion-exchange chromatography
Separates acid-labile amino acids under basic pH conditions
Separates strongly acidic amino acids
Requires OPA or ninhydrin postcolumn derivatization
The anion-exchange selectivity of the AminoPac PA1 can be used to resolve peaks that cannot be resolved using a cation-exchange column. For example, O-phosphorylated amino acids are resolved and eluted near the end of a hydrolysate chromatogram. Arogenic acid, a precursor to tyrosine and phenylalanine, decomposes under the acidic conditions required for cation-exchange separation or PITC derivatization, but it is stable in alkaline eluents used with AminoPac PA1 column.
The PA1 is particularly well suited for acidic and acid-labile amino acids and for amino acid pairs not compley resolved by cation-exchange chromatography.
| AminoPac PA1 Column for Specialized Amino Acid Applications with Postcolumn Derivatization Specifications | |
|---|---|
| Dimensions | AminoPac PA1 Analytical Column: 4 × 250 mm AminoPac PA1 Guard Column: 4 × 50 mm |
| Maximum operating pressure | 4000 psi (27.9 MPa) |
| Recommended operating temperature | Ambient |
| Typical operating conditions | 1.0 mL/min gradient Recommended flow rate: 1.0 mL/min |
| phase compatibility | Compatible with up to 5% common organic solvents. Recommended eluents: hydroxide, borate, and acetate. |
| Resin composition | 10-µm-diameter, pellicular substrate (2% cross-linked) agglomerated with a 180 nm MicroBead alkyl quaternary ammonium functionalized latex (20% cross-linked). |
| Anion-exchange capacity | N/A |
| Column construction | PEEK with 10-32 threaded ferrule-style end fittings. All components are nonmetallic. |