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4.12. Transwell Assay

Transwell assays were performed using transwell chambers with or without Matrigel coating to detect cell invasion and migration ability, respectively. For invasion assay, Matrigel (082706, ABW, China) was dissolved with a serum-free medium at 1:8, and 100 µL was added to each upper chamber. Then, 200 µL serum-free medium containing 5 × 104 was added to the upper chamber, while the lower chamber was added with 600 µL 10% FBS medium. The cells were fixed and stained after 24 h of incubation. For migration assay, 200 µL serum-free medium containing 2.5 × 104 was added to the upper chamber, while the lower chamber was added with 800 µL 10% FBS medium. The next procedures are the same as for the invasion assay. Three randomly selected fields of view were photographed under the microscope and the images were counted using ImageJ software (National Institutes of Health).

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