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Transwell Invasion Assay

A Transwell chamber with an 8-μm pore polycarbonate membrane (Biofil, Guangzhou, China) was used for the invasion  assay. A medium containing 10% fetal bovine serum was added to the lower chamber. The cells were suspended in  a serum-free medium and inoculated into the upper chamber coated with a matrix gel (ABW, Shanghai, China). After  incubation for 24 h, the cells in the upper chamber were scraped with a cotton swab, fixed on the lower surface of the  membrane with paraformaldehyde for 30 min, and stained with crystal violet (Solarbio) for 30 min. The cells were  counted after capturing the images.

Tube Formation Assay 

The 96-well plates coated with matrix gel (ABW) were incubated at 37 °C in 5% CO2. After 30 min, 50 μL of cell  suspension was added to each well. Images were captured after 4 h.

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